CD46 facilitates entry and dissemination of human cytomegalovirus

Kathryn R. Stein, Thomas J. Gardner, Rosmel E. Hernandez, Thomas A. Kraus, James A. Duty, Iban Ubarretxena-Belandia, Thomas M. Moran and Domenico Tortorella ()
Additional contact information
Kathryn R. Stein: Icahn School of Medicine at Mount Sinai
Thomas J. Gardner: Icahn School of Medicine at Mount Sinai
Rosmel E. Hernandez: Icahn School of Medicine at Mount Sinai
Thomas A. Kraus: Icahn School of Medicine at Mount Sinai
James A. Duty: Icahn School of Medicine at Mount Sinai
Iban Ubarretxena-Belandia: Icahn School of Medicine at Mount Sinai
Thomas M. Moran: Icahn School of Medicine at Mount Sinai
Domenico Tortorella: Icahn School of Medicine at Mount Sinai

Nature Communications, 2019, vol. 10, issue 1, 1-13

Abstract: Abstract Human cytomegalovirus (CMV) causes a wide array of disease to diverse populations of immune-compromised individuals. Thus, a more comprehensive understanding of how CMV enters numerous host cell types is necessary to further delineate the complex nature of CMV pathogenesis and to develop targeted therapeutics. To that end, we establish a vaccination strategy utilizing membrane vesicles derived from epithelial cells to generate a library of monoclonal antibodies (mAbs) targeting cell surface proteins in their native conformation. A high-throughput inhibition assay is employed to screen these antibodies for their ability to limit infection, and mAbs targeting CD46 are identified. In addition, a significant reduction of viral proliferation in CD46-KO epithelial cells confirms a role for CD46 function in viral dissemination. Further, we demonstrate a CD46-dependent entry pathway of virus infection in trophoblasts, but not in fibroblasts, highlighting the complexity of CMV entry and identifying CD46 as an entry factor in congenital infection.

Date: 2019
References: Add references at CitEc
Citations:

Downloads: (external link)
https://www.nature.com/articles/s41467-019-10587-1 Abstract (text/html)

Related works:
This item may be available elsewhere in EconPapers: Search for items with the same title.

Export reference: BibTeX RIS (EndNote, ProCite, RefMan) HTML/Text

Persistent link: https://EconPapers.repec.org/RePEc:nat:natcom:v:10:y:2019:i:1:d:10.1038_s41467-019-10587-1

Ordering information: This journal article can be ordered from
https://www.nature.com/ncomms/

DOI: 10.1038/s41467-019-10587-1

Access Statistics for this article

Nature Communications is currently edited by Nathalie Le Bot, Enda Bergin and Fiona Gillespie

More articles in Nature Communications from Nature
Bibliographic data for series maintained by Sonal Shukla () and Springer Nature Abstracting and Indexing ().