Addendum: Precise tuning of gene expression levels in mammalian cells

Yale S. Michaels, Mike B. Barnkob, Hector Barbosa, Toni A. Baeumler, Mary K. Thompson, Violaine Andre, Huw Colin-York, Marco Fritzsche, Uzi Gileadi, Hilary M. Sheppard, David J. H. F. Knapp, Thomas A. Milne, Vincenzo Cerundolo and Tudor A. Fulga ()
Additional contact information
Yale S. Michaels: University of Oxford
Mike B. Barnkob: Weatherall Institute of Molecular Medicine University of Oxford
Hector Barbosa: University of Oxford
Toni A. Baeumler: University of Oxford
Mary K. Thompson: University of Oxford
Violaine Andre: Weatherall Institute of Molecular Medicine University of Oxford
Huw Colin-York: Weatherall Institute of Molecular Medicine University of Oxford
Marco Fritzsche: Weatherall Institute of Molecular Medicine University of Oxford
Uzi Gileadi: Weatherall Institute of Molecular Medicine University of Oxford
Hilary M. Sheppard: University of Auckland
David J. H. F. Knapp: University of Oxford
Thomas A. Milne: University of Oxford
Vincenzo Cerundolo: Weatherall Institute of Molecular Medicine University of Oxford
Tudor A. Fulga: University of Oxford

Nature Communications, 2019, vol. 10, issue 1, 1-3

Abstract: Following re-sequencing of the miSFIT constructs used in the paper, two of the construct variants inserted into the 3’UTR of PD-1, namely ‘12C’ and ‘17A, 18G’, have been found to contain additional insertions not present in the other construct variants. The data points corresponding to these constructs in Figs. 2c, f and Supplementary Fig. 9 are therefore no longer valid. However the overall conclusion that step-wise control over gene expression levels using the miSFIT constructs remains unaffected by these errors. Updated versions of Fig. 2 and Supplementary Fig. 9 are presented in the accompanying Addendum.

Date: 2019
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DOI: 10.1038/s41467-019-10615-0

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