Fast genetic mapping of complex traits in C. elegans using millions of individuals in bulk

Alejandro Burga (), Eyal Ben-David (), Tzitziki Lemus Vergara, James Boocock and Leonid Kruglyak ()
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Alejandro Burga: University of California, Los Angeles
Eyal Ben-David: University of California, Los Angeles
Tzitziki Lemus Vergara: University of California, Los Angeles
James Boocock: University of California, Los Angeles
Leonid Kruglyak: University of California, Los Angeles

Nature Communications, 2019, vol. 10, issue 1, 1-10

Abstract: Abstract Genetic studies of complex traits in animals have been hindered by the need to generate, maintain, and phenotype large panels of recombinant lines. We developed a new method, C. elegans eXtreme Quantitative Trait Locus (ceX-QTL) mapping, that overcomes this obstacle via bulk selection on millions of unique recombinant individuals. We use ceX-QTL to map a drug resistance locus with high resolution. We also map differences in gene expression in live worms and discovered that mutations in the co-chaperone sti-1 upregulate the transcription of HSP-90. Lastly, we use ceX-QTL to map loci that influence fitness genome-wide confirming previously reported causal variants and uncovering new fitness loci. ceX-QTL is fast, powerful and cost-effective, and will accelerate the study of complex traits in animals.

Date: 2019
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DOI: 10.1038/s41467-019-10636-9

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