Conformational proofreading of distant 40S ribosomal subunit maturation events by a long-range communication mechanism

Valentin Mitterer, Ramtin Shayan, Sébastien Ferreira-Cerca (), Guillaume Murat, Tanja Enne, Dana Rinaldi, Sarah Weigl, Hajrija Omanic, Pierre-Emmanuel Gleizes, Dieter Kressler (), Celia Plisson-Chastang () and Brigitte Pertschy ()
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Valentin Mitterer: University of Graz
Ramtin Shayan: Centre de Biologie Intégrative, Université de Toulouse, CNRS, UPS
Sébastien Ferreira-Cerca: Genetics and Microbiology, University of Regensburg
Guillaume Murat: University of Fribourg
Tanja Enne: University of Graz
Dana Rinaldi: Centre de Biologie Intégrative, Université de Toulouse, CNRS, UPS
Sarah Weigl: University of Graz
Hajrija Omanic: University of Graz
Pierre-Emmanuel Gleizes: Centre de Biologie Intégrative, Université de Toulouse, CNRS, UPS
Dieter Kressler: University of Fribourg
Celia Plisson-Chastang: Centre de Biologie Intégrative, Université de Toulouse, CNRS, UPS
Brigitte Pertschy: University of Graz

Nature Communications, 2019, vol. 10, issue 1, 1-15

Abstract: Abstract Eukaryotic ribosomes are synthesized in a hierarchical process driven by a plethora of assembly factors, but how maturation events at physically distant sites on pre-ribosomes are coordinated is poorly understood. Using functional analyses and cryo-EM, we show that ribosomal protein Rps20 orchestrates communication between two multi-step maturation events across the pre-40S subunit. Our study reveals that during pre-40S maturation, formation of essential contacts between Rps20 and Rps3 permits assembly factor Ltv1 to recruit the Hrr25 kinase, thereby promoting Ltv1 phosphorylation. In parallel, a deeply buried Rps20 loop reaches to the opposite pre-40S side, where it stimulates Rio2 ATPase activity. Both cascades converge to the final maturation steps releasing Rio2 and phosphorylated Ltv1. We propose that conformational proofreading exerted via Rps20 constitutes a checkpoint permitting assembly factor release and progression of pre-40S maturation only after completion of all earlier maturation steps.

Date: 2019
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DOI: 10.1038/s41467-019-10678-z

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