DNA double-strand breaks in telophase lead to coalescence between segregated sister chromatid loci

Jessel Ayra-Plasencia and Félix Machín ()
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Jessel Ayra-Plasencia: Hospital Universitario Nuestra Señora de Candelaria
Félix Machín: Hospital Universitario Nuestra Señora de Candelaria

Nature Communications, 2019, vol. 10, issue 1, 1-14

Abstract: Abstract DNA double strand breaks (DSBs) pose a high risk for genome integrity. Cells repair DSBs through homologous recombination (HR) when a sister chromatid is available. HR is upregulated by the cycling dependent kinase (CDK) despite the paradox of telophase, where CDK is high but a sister chromatid is not nearby. Here we study in the budding yeast the response to DSBs in telophase, and find they activate the DNA damage checkpoint (DDC), leading to a telophase-to-G1 delay. Outstandingly, we observe a partial reversion of sister chromatid segregation, which includes approximation of segregated material, de novo formation of anaphase bridges, and coalescence between sister loci. We finally show that DSBs promote a massive change in the dynamics of telophase microtubules (MTs), together with dephosphorylation and relocalization of kinesin-5 Cin8. We propose that chromosome segregation is not irreversible and that DSB repair using the sister chromatid is possible in telophase.

Date: 2019
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Persistent link: https://EconPapers.repec.org/RePEc:nat:natcom:v:10:y:2019:i:1:d:10.1038_s41467-019-10742-8

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DOI: 10.1038/s41467-019-10742-8

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