Site-specific cleavage of bacterial MucD by secreted proteases mediates antibacterial resistance in Arabidopsis

Yiming Wang, Ruben Garrido-Oter, Jingni Wu, Thomas M. Winkelmüller, Matthew Agler, Thomas Colby, Tatsuya Nobori, Eric Kemen and Kenichi Tsuda ()
Additional contact information
Yiming Wang: Max Planck Institute for Plant Breeding Research
Ruben Garrido-Oter: Max Planck Institute for Plant Breeding Research
Jingni Wu: Max Planck Institute for Plant Breeding Research
Thomas M. Winkelmüller: Max Planck Institute for Plant Breeding Research
Matthew Agler: Max Planck Institute for Plant Breeding Research
Thomas Colby: Max Planck Institute for Plant Breeding Research
Tatsuya Nobori: Max Planck Institute for Plant Breeding Research
Eric Kemen: Max Planck Institute for Plant Breeding Research
Kenichi Tsuda: Max Planck Institute for Plant Breeding Research

Nature Communications, 2019, vol. 10, issue 1, 1-12

Abstract: Abstract Plant innate immunity restricts growth of bacterial pathogens that threaten global food security. However, the mechanisms by which plant immunity suppresses bacterial growth remain enigmatic. Here we show that Arabidopsis thaliana secreted aspartic protease 1 and 2 (SAP1 and SAP2) cleave the evolutionarily conserved bacterial protein MucD to redundantly inhibit the growth of the bacterial pathogen Pseudomonas syringae. Antibacterial activity of SAP1 requires its protease activity in planta and in vitro. Plants overexpressing SAP1 exhibit enhanced MucD cleavage and resistance but incur no penalties in growth and reproduction, while sap1 sap2 double mutant plants exhibit compromised MucD cleavage and resistance against P. syringae. P. syringae lacking mucD shows compromised growth in planta and in vitro. Notably, growth of ΔmucD complemented with the non-cleavable MucDF106Y is not affected by SAP activity in planta and in vitro. Our findings identify the genetic factors and biochemical process underlying an antibacterial mechanism in plants.

Date: 2019
References: Add references at CitEc
Citations:

Downloads: (external link)
https://www.nature.com/articles/s41467-019-10793-x Abstract (text/html)

Related works:
This item may be available elsewhere in EconPapers: Search for items with the same title.

Export reference: BibTeX RIS (EndNote, ProCite, RefMan) HTML/Text

Persistent link: https://EconPapers.repec.org/RePEc:nat:natcom:v:10:y:2019:i:1:d:10.1038_s41467-019-10793-x

Ordering information: This journal article can be ordered from
https://www.nature.com/ncomms/

DOI: 10.1038/s41467-019-10793-x

Access Statistics for this article

Nature Communications is currently edited by Nathalie Le Bot, Enda Bergin and Fiona Gillespie

More articles in Nature Communications from Nature
Bibliographic data for series maintained by Sonal Shukla () and Springer Nature Abstracting and Indexing ().