Bone-targeting AAV-mediated silencing of Schnurri-3 prevents bone loss in osteoporosis

Yeon-Suk Yang, Jun Xie, Dan Wang, Jung-Min Kim, Phillip W. L. Tai, Ellen Gravallese, Guangping Gao () and Jae-Hyuck Shim ()
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Yeon-Suk Yang: University of Massachusetts Medical School
Jun Xie: University of Massachusetts Medical School
Dan Wang: University of Massachusetts Medical School
Jung-Min Kim: University of Massachusetts Medical School
Phillip W. L. Tai: University of Massachusetts Medical School
Ellen Gravallese: University of Massachusetts Medical School
Guangping Gao: University of Massachusetts Medical School
Jae-Hyuck Shim: University of Massachusetts Medical School

Nature Communications, 2019, vol. 10, issue 1, 1-13

Abstract: Abstract RNAi-based bone anabolic gene therapy has demonstrated initial success, but many practical challenges are still unmet. Here, we demonstrate that a recombinant adeno-associated virus 9 (rAAV9) is highly effective for transducing osteoblast lineage cells in the bone. The adaptor protein Schnurri-3 (SHN3) is a promising therapeutic target for osteoporosis, as deletion of shn3 prevents bone loss in osteoporotic mice and short-term inhibition of shn3 in adult mice increases bone mass. Accordingly, systemic and direct joint administration of an rAAV9 vector carrying an artificial-microRNA that targets shn3 (rAAV9-amiR-shn3) in mice markedly enhanced bone formation via augmented osteoblast activity. Additionally, systemic delivery of rAAV9-amiR-shn3 in osteoporotic mice counteracted bone loss and enhanced bone mechanical properties. Finally, we rationally designed a capsid that exhibits improved specificity to bone by grafting the bone-targeting peptide motif (AspSerSer)6 onto the AAV9-VP2 capsid protein. Collectively, our results identify a bone-targeting rAAV-mediated gene therapy for osteoporosis.

Date: 2019
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DOI: 10.1038/s41467-019-10809-6

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