A genetically encoded probe for imaging nascent and mature HA-tagged proteins in vivo
Ning Zhao,
Kouta Kamijo,
Philip D. Fox,
Haruka Oda,
Tatsuya Morisaki,
Yuko Sato,
Hiroshi Kimura and
Timothy J. Stasevich ()
Additional contact information
Ning Zhao: Colorado State University
Kouta Kamijo: Tokyo Institute of Technology
Philip D. Fox: Colorado State University
Haruka Oda: Tokyo Institute of Technology
Tatsuya Morisaki: Colorado State University
Yuko Sato: Tokyo Institute of Technology
Hiroshi Kimura: Tokyo Institute of Technology
Timothy J. Stasevich: Colorado State University
Nature Communications, 2019, vol. 10, issue 1, 1-16
Abstract:
Abstract To expand the toolbox of imaging in living cells, we have engineered a single-chain variable fragment binding the linear HA epitope with high affinity and specificity in vivo. The resulting probe, called the HA frankenbody, can light up in multiple colors HA-tagged nuclear, cytoplasmic, membrane, and mitochondrial proteins in diverse cell types. The HA frankenbody also enables state-of-the-art single-molecule experiments in living cells, which we demonstrate by tracking single HA-tagged histones in U2OS cells and single mRNA translation dynamics in both U2OS cells and neurons. Together with the SunTag, we also track two mRNA species simultaneously to demonstrate comparative single-molecule studies of translation can now be done with genetically encoded tools alone. Finally, we use the HA frankenbody to precisely quantify the expression of HA-tagged proteins in developing zebrafish embryos. The versatility of the HA frankenbody makes it a powerful tool for imaging protein dynamics in vivo.
Date: 2019
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Persistent link: https://EconPapers.repec.org/RePEc:nat:natcom:v:10:y:2019:i:1:d:10.1038_s41467-019-10846-1
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DOI: 10.1038/s41467-019-10846-1
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