A split fluorescent reporter with rapid and reversible complementation

Alison G. Tebo and Arnaud Gautier ()
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Alison G. Tebo: CNRS
Arnaud Gautier: CNRS

Nature Communications, 2019, vol. 10, issue 1, 1-8

Abstract: Abstract Interactions between proteins play an essential role in metabolic and signaling pathways, cellular processes and organismal systems. We report the development of splitFAST, a fluorescence complementation system for the visualization of transient protein-protein interactions in living cells. Engineered from the fluorogenic reporter FAST (Fluorescence-Activating and absorption-Shifting Tag), which specifically and reversibly binds fluorogenic hydroxybenzylidene rhodanine (HBR) analogs, splitFAST displays rapid and reversible complementation, allowing the real-time visualization of both the formation and the dissociation of a protein assembly.

Date: 2019
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DOI: 10.1038/s41467-019-10855-0

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