Tethering of vesicles to the Golgi by GMAP210 controls LAT delivery to the immune synapse

Andres Ernesto Zucchetti, Laurence Bataille, Jean-Marie Carpier, Stéphanie Dogniaux, Mabel San Roman-Jouve, Mathieu Maurin, Michael W. Stuck, Rosa M. Rios, Cosima T. Baldari, Gregory J. Pazour and Claire Hivroz ()
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Andres Ernesto Zucchetti: Integrative analysis of T cell activation team
Laurence Bataille: Integrative analysis of T cell activation team
Jean-Marie Carpier: Integrative analysis of T cell activation team
Stéphanie Dogniaux: Integrative analysis of T cell activation team
Mabel San Roman-Jouve: Integrative analysis of T cell activation team
Mathieu Maurin: Integrative analysis of T cell activation team
Michael W. Stuck: University of Massachusetts Medical School
Rosa M. Rios: CABIMER-CSIC/US/UPO
Cosima T. Baldari: University of Siena
Gregory J. Pazour: University of Massachusetts Medical School
Claire Hivroz: Integrative analysis of T cell activation team

Nature Communications, 2019, vol. 10, issue 1, 1-17

Abstract: Abstract The T cell immune synapse is a site of intense vesicular trafficking. Here we show that the golgin GMAP210, known to capture vesicles and organize membrane traffic at the Golgi, is involved in the vesicular transport of LAT to the immune synapse. Upon activation, more GMAP210 interact with LAT-containing vesicles and go together with LAT to the immune synapse. Regulating LAT recruitment and LAT-dependent signaling, GMAP210 controls T cell activation. Using a rerouting and capture assay, we show that GMAP210 captures VAMP7-decorated vesicles. Overexpressing different domains of GMAP210, we also show that GMAP210 allows their specific delivery to the immune synapse by tethering LAT-vesicles to the Golgi. Finally, in a model of ectopic expression of LAT in ciliated cells, we show that GMAP210 tethering activity controls the delivery of LAT to the cilium. Hence, our results reveal a function for the golgin GMAP210 conveying specific vesicles to the immune synapse.

Date: 2019
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DOI: 10.1038/s41467-019-10891-w

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