Dual-color fluorescent nanoparticles showing perfect color-specific photoswitching for bioimaging and super-resolution microscopy

Dojin Kim, Keunsoo Jeong, Ji Eon Kwon, Hyeonjong Park, Seokyung Lee, Sehoon Kim and Soo Young Park ()
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Dojin Kim: Seoul National University
Keunsoo Jeong: Korea Institute of Science and Technology
Ji Eon Kwon: Seoul National University
Hyeonjong Park: Korea Institute of Science and Technology
Seokyung Lee: Korea Institute of Science and Technology
Sehoon Kim: Korea Institute of Science and Technology
Soo Young Park: Seoul National University

Nature Communications, 2019, vol. 10, issue 1, 1-10

Abstract: Abstract Dual-emissive systems showing color-specific photoswitching are promising in bioimaging and super-resolution microscopy. However, their switching efficiency has been limited because a delicate manipulation of all the energy transfer crosstalks in the systems is unfeasible. Here, we report a perfect color-specific photoswitching, which is rationally designed by combining the complete off-to-on fluorescence switching capability of a fluorescent photochromic diarylethene and the frustrated energy transfer to the other fluorescent dye based on the excited-state intramolecular proton transfer (ESIPT) process. Upon alternation of UV and visible light irradiations, the system achieves 100% switching on/off of blue emission from the diarylethene while orange emission from the ESIPT dye is unchanged in the polymer film. By fabricating this system into biocompatible polymer nanoparticles, we demonstrate microscopic imaging of RAW264.7 macrophage cells with reversible blue-color specific fluorescence switching that enables super-resolution imaging with a resolution of 70 nm.

Date: 2019
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DOI: 10.1038/s41467-019-10986-4

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