Dissecting a heterotic gene through GradedPool-Seq mapping informs a rice-improvement strategy

Changsheng Wang, Shican Tang, Qilin Zhan, Qingqing Hou, Yan Zhao, Qiang Zhao, Qi Feng, Congcong Zhou, Danfeng Lyu, Lingling Cui, Yan Li, Jiashun Miao, Chuanrang Zhu, Yiqi Lu, Yongchun Wang, Ziqun Wang, Jingjie Zhu, Yingying Shangguan, Junyi Gong, Shihua Yang, Wuqi Wang, Jianfu Zhang, Huaan Xie, Xuehui Huang () and Bin Han ()
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Changsheng Wang: Chinese Academy of Sciences
Shican Tang: Chinese Academy of Sciences
Qilin Zhan: Chinese Academy of Sciences
Qingqing Hou: Chinese Academy of Sciences
Yan Zhao: Chinese Academy of Sciences
Qiang Zhao: Chinese Academy of Sciences
Qi Feng: Chinese Academy of Sciences
Congcong Zhou: Chinese Academy of Sciences
Danfeng Lyu: Chinese Academy of Sciences
Lingling Cui: Chinese Academy of Sciences
Yan Li: Chinese Academy of Sciences
Jiashun Miao: Chinese Academy of Sciences
Chuanrang Zhu: Chinese Academy of Sciences
Yiqi Lu: Chinese Academy of Sciences
Yongchun Wang: Chinese Academy of Sciences
Ziqun Wang: Chinese Academy of Sciences
Jingjie Zhu: Chinese Academy of Sciences
Yingying Shangguan: Chinese Academy of Sciences
Junyi Gong: Chinese Academy of Agricultural Sciences
Shihua Yang: Chinese Academy of Agricultural Sciences
Wuqi Wang: Fujian Academy of Agricultural Sciences
Jianfu Zhang: Fujian Academy of Agricultural Sciences
Huaan Xie: Fujian Academy of Agricultural Sciences
Xuehui Huang: Shanghai Normal University
Bin Han: Chinese Academy of Sciences

Nature Communications, 2019, vol. 10, issue 1, 1-12

Abstract: Abstract Hybrid rice breeding for exploiting hybrid vigor, heterosis, has greatly increased grain yield. However, the heterosis-related genes associated with rice grain production remain largely unknown, partly because comprehensive mapping of heterosis-related traits is still labor-intensive and time-consuming. Here, we present a quantitative trait locus (QTL) mapping method, GradedPool-Seq, for rapidly mapping QTLs by whole-genome sequencing of graded-pool samples from F2 progeny via bulked-segregant analysis. We implement this method and map-based cloning to dissect the heterotic QTL GW3p6 from the female line. We then generate the near isogenic line NIL-FH676::GW3p6 by introgressing the GW3p6 allele from the female line Guangzhan63-4S into the male inbred line Fuhui676. The NIL-FH676::GW3p6 exhibits grain yield highly increased compared to Fuhui676. This study demonstrates that it may be possible to achieve a high level of grain production in inbred rice lines without the need to construct hybrids.

Date: 2019
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DOI: 10.1038/s41467-019-11017-y

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