Label-free neuroimaging in vivo using synchronous angular scanning microscopy with single-scattering accumulation algorithm

Moonseok Kim, Yonghyeon Jo, Jin Hee Hong, Suhyun Kim, Seokchan Yoon, Kyung-Deok Song, Sungsam Kang, Byunghak Lee, Guang Hoon Kim, Hae-Chul Park and Wonshik Choi ()
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Moonseok Kim: Institute for Basic Science
Yonghyeon Jo: Institute for Basic Science
Jin Hee Hong: Institute for Basic Science
Suhyun Kim: Korea University
Seokchan Yoon: Institute for Basic Science
Kyung-Deok Song: Institute for Basic Science
Sungsam Kang: Massachusetts Institute of Technology
Byunghak Lee: Korea Electrotechnology Research Institute
Guang Hoon Kim: Korea Electrotechnology Research Institute
Hae-Chul Park: Korea University
Wonshik Choi: Institute for Basic Science

Nature Communications, 2019, vol. 10, issue 1, 1-9

Abstract: Abstract Label-free in vivo imaging is crucial for elucidating the underlying mechanisms of many important biological systems in their most native states. However, the applicability of existing modalities has been limited to either superficial layers or early developmental stages due to tissue turbidity. Here, we report a synchronous angular scanning microscope for the rapid interferometric recording of the time-gated reflection matrix, which is a unique matrix characterizing full light-specimen interaction. By applying single scattering accumulation algorithm to the recorded matrix, we removed both high-order sample-induced aberrations and multiple scattering noise with the effective aberration correction speed of 10,000 modes/s. We demonstrated in vivo imaging of whole neural network throughout the hindbrain of the larval zebrafish at a matured stage where physical dissection used to be required for conventional imaging. Our method will expand the scope of applications for optical imaging, where fully non-invasive interrogation of living specimens is critical.

Date: 2019
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DOI: 10.1038/s41467-019-11040-z

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