Structural underpinnings of Ric8A function as a G-protein α-subunit chaperone and guanine-nucleotide exchange factor

Dhiraj Srivastava, Lokesh Gakhar and Nikolai O. Artemyev ()
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Dhiraj Srivastava: University of Iowa Carver College of Medicine
Lokesh Gakhar: University of Iowa Carver College of Medicine
Nikolai O. Artemyev: University of Iowa Carver College of Medicine

Nature Communications, 2019, vol. 10, issue 1, 1-14

Abstract: Abstract Resistance to inhibitors of cholinesterase 8A (Ric8A) is an essential regulator of G protein α-subunits (Gα), acting as a guanine nucleotide exchange factor and a chaperone. We report two crystal structures of Ric8A, one in the apo form and the other in complex with a tagged C-terminal fragment of Gα. These structures reveal two principal domains of Ric8A: an armadillo-fold core and a flexible C-terminal tail. Additionally, they show that the Gα C-terminus binds to a highly-conserved patch on the concave surface of the Ric8A armadillo-domain, with selectivity determinants residing in the Gα sequence. Biochemical analysis shows that the Ric8A C-terminal tail is critical for its stability and function. A model of the Ric8A/Gα complex derived from crosslinking mass spectrometry and molecular dynamics simulations suggests that the Ric8A C-terminal tail helps organize the GTP-binding site of Gα. This study lays the groundwork for understanding Ric8A function at the molecular level.

Date: 2019
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DOI: 10.1038/s41467-019-11088-x

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