The late stage of COPI vesicle fission requires shorter forms of phosphatidic acid and diacylglycerol

Seung-Yeol Park, Jia-Shu Yang, Zhen Li, Pan Deng, Xiaohong Zhu, David Young, Maria Ericsson, Ruben L. H. Andringa, Adriaan J. Minnaard, Chunmei Zhu, Fei Sun, D. Branch Moody, Andrew J. Morris, Jun Fan and Victor W. Hsu ()
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Seung-Yeol Park: Harvard Medical School
Jia-Shu Yang: Harvard Medical School
Zhen Li: City University of Hong Kong
Pan Deng: University of Kentucky
Xiaohong Zhu: City University of Hong Kong
David Young: Harvard Medical School
Maria Ericsson: Harvard Medical School
Ruben L. H. Andringa: University of Groningen
Adriaan J. Minnaard: University of Groningen
Chunmei Zhu: Chinese Academy of Sciences
Fei Sun: Chinese Academy of Sciences
D. Branch Moody: Harvard Medical School
Andrew J. Morris: University of Kentucky
Jun Fan: City University of Hong Kong
Victor W. Hsu: Harvard Medical School

Nature Communications, 2019, vol. 10, issue 1, 1-14

Abstract: Abstract Studies on vesicle formation by the Coat Protein I (COPI) complex have contributed to a basic understanding of how vesicular transport is initiated. Phosphatidic acid (PA) and diacylglycerol (DAG) have been found previously to be required for the fission stage of COPI vesicle formation. Here, we find that PA with varying lipid geometry can all promote early fission, but only PA with shortened acyl chains promotes late fission. Moreover, diacylglycerol (DAG) acts after PA in late fission, with this role of DAG also requiring shorter acyl chains. Further highlighting the importance of the short-chain lipid geometry for late fission, we find that shorter forms of PA and DAG promote the vesiculation ability of COPI fission factors. These findings advance a general understanding of how lipid geometry contributes to membrane deformation for vesicle fission, and also how proteins and lipids coordinate their actions in driving this process.

Date: 2019
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DOI: 10.1038/s41467-019-11324-4

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