Robust continuous in vitro culture of the Plasmodium cynomolgi erythrocytic stages

Adeline C. Y. Chua, Jessica Jie Ying Ong, Benoit Malleret, Rossarin Suwanarusk, Varakorn Kosaisavee, Anne-Marie Zeeman, Caitlin A. Cooper, Kevin S. W. Tan, Rou Zhang, Bee Huat Tan, Siti Nurdiana Abas, Andy Yip, Anne Elliot, Chester J. Joyner, Jee Sun Cho, Kate Breyer, Szczepan Baran, Amber Lange, Steven P. Maher, François Nosten, Christophe Bodenreider, Bryan K. S. Yeung, Dominique Mazier, Mary R. Galinski, Nathalie Dereuddre-Bosquet, Roger Grand, Clemens H. M. Kocken, Laurent Rénia, Dennis E. Kyle, Thierry T. Diagana, Georges Snounou, Bruce Russell () and Pablo Bifani ()
Additional contact information
Adeline C. Y. Chua: Singapore Immunology Network, A*STAR
Jessica Jie Ying Ong: University of Otago
Benoit Malleret: Singapore Immunology Network, A*STAR
Rossarin Suwanarusk: Singapore Immunology Network, A*STAR
Varakorn Kosaisavee: National University of Singapore
Anne-Marie Zeeman: Biomedical Primate Research Centre
Caitlin A. Cooper: University of Georgia
Kevin S. W. Tan: National University of Singapore
Rou Zhang: National University of Singapore
Bee Huat Tan: Novartis Institute for Tropical Diseases
Siti Nurdiana Abas: Novartis Institute for Tropical Diseases
Andy Yip: Novartis Institute for Tropical Diseases
Anne Elliot: University of Georgia
Chester J. Joyner: Emory University
Jee Sun Cho: National University of Singapore
Kate Breyer: Novartis Institutes for Biomedical Research
Szczepan Baran: Novartis Institutes for Biomedical Research
Amber Lange: Novartis Institutes for Biomedical Research
Steven P. Maher: University of Georgia
François Nosten: Mahidol University
Christophe Bodenreider: Novartis Institute for Tropical Diseases
Bryan K. S. Yeung: Novartis Institute for Tropical Diseases
Dominique Mazier: Sorbonne Universités, UPMC Univ Paris 06, CR7, Centre d’Immunologie et des Maladies Infectieuses (CIMI-Paris)
Mary R. Galinski: Emory University
Nathalie Dereuddre-Bosquet: CEA-Université Paris Sud 11-INSERM U1184, Immunology of Viral Infections and Autoimmune Diseases (IMVA), IDMIT Department
Roger Grand: CEA-Université Paris Sud 11-INSERM U1184, Immunology of Viral Infections and Autoimmune Diseases (IMVA), IDMIT Department
Clemens H. M. Kocken: Biomedical Primate Research Centre
Laurent Rénia: Singapore Immunology Network, A*STAR
Dennis E. Kyle: University of Georgia
Thierry T. Diagana: Novartis Institute for Tropical Diseases
Georges Snounou: Sorbonne Universités, UPMC Univ Paris 06, CR7, Centre d’Immunologie et des Maladies Infectieuses (CIMI-Paris)
Bruce Russell: University of Otago
Pablo Bifani: Singapore Immunology Network, A*STAR

Nature Communications, 2019, vol. 10, issue 1, 1-13

Abstract: Abstract The ability to culture pathogenic organisms substantially enhances the quest for fundamental knowledge and the development of vaccines and drugs. Thus, the elaboration of a protocol for the in vitro cultivation of the erythrocytic stages of Plasmodium falciparum revolutionized research on this important parasite. However, for P. vivax, the most widely distributed and difficult to treat malaria parasite, a strict preference for reticulocytes thwarts efforts to maintain it in vitro. Cultivation of P. cynomolgi, a macaque-infecting species phylogenetically close to P. vivax, was briefly reported in the early 1980s, but not pursued further. Here, we define the conditions under which P. cynomolgi can be adapted to long term in vitro culture to yield parasites that share many of the morphological and phenotypic features of P. vivax. We further validate the potential of this culture system for high-throughput screening to prime and accelerate anti-P. vivax drug discovery efforts.

Date: 2019
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DOI: 10.1038/s41467-019-11332-4

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