Single-molecule localization microscopy and tracking with red-shifted states of conventional BODIPY conjugates in living cells

Santosh Adhikari, Joe Moscatelli, Elizabeth M. Smith, Chiranjib Banerjee and Elias M. Puchner ()
Additional contact information
Santosh Adhikari: School of Physics and Astronomy University of Minnesota, Twin Cities Physics and Nanotechnology (PAN)
Joe Moscatelli: School of Physics and Astronomy University of Minnesota, Twin Cities Physics and Nanotechnology (PAN)
Elizabeth M. Smith: School of Physics and Astronomy University of Minnesota, Twin Cities Physics and Nanotechnology (PAN)
Chiranjib Banerjee: School of Physics and Astronomy University of Minnesota, Twin Cities Physics and Nanotechnology (PAN)
Elias M. Puchner: School of Physics and Astronomy University of Minnesota, Twin Cities Physics and Nanotechnology (PAN)

Nature Communications, 2019, vol. 10, issue 1, 1-12

Abstract: Abstract Single-molecule localization microscopy (SMLM) is a rapidly evolving technique to resolve subcellular structures and single-molecule dynamics at the nanoscale. Here, we employ conventional BODIPY conjugates for live-cell SMLM via their previously reported red-shifted ground-state dimers (DII), which transiently form through bi-molecular encounters and emit bright single-molecule fluorescence. We employ the versatility of DII-state SMLM to resolve the nanoscopic spatial regulation and dynamics of single fatty acid analogs (FAas) and lipid droplets (LDs) in living yeast and mammalian cells with two colors. In fed cells, FAas localize to the endoplasmic reticulum and LDs of ~125 nm diameter. Upon fasting, however, FAas form dense, non-LD clusters of ~100 nm diameter at the plasma membrane and transition from free diffusion to confined immobilization. Our reported SMLM capability of conventional BODIPY conjugates is further demonstrated by imaging lysosomes in mammalian cells and enables simple and versatile live-cell imaging of sub-cellular structures at the nanoscale.

Date: 2019
References: Add references at CitEc
Citations:

Downloads: (external link)
https://www.nature.com/articles/s41467-019-11384-6 Abstract (text/html)

Related works:
This item may be available elsewhere in EconPapers: Search for items with the same title.

Export reference: BibTeX RIS (EndNote, ProCite, RefMan) HTML/Text

Persistent link: https://EconPapers.repec.org/RePEc:nat:natcom:v:10:y:2019:i:1:d:10.1038_s41467-019-11384-6

Ordering information: This journal article can be ordered from
https://www.nature.com/ncomms/

DOI: 10.1038/s41467-019-11384-6

Access Statistics for this article

Nature Communications is currently edited by Nathalie Le Bot, Enda Bergin and Fiona Gillespie

More articles in Nature Communications from Nature
Bibliographic data for series maintained by Sonal Shukla () and Springer Nature Abstracting and Indexing ().