A CRISPR-Cas12a-derived biosensing platform for the highly sensitive detection of diverse small molecules

Mindong Liang, Zilong Li, Weishan Wang, Jiakun Liu, Leshi Liu, Guoliang Zhu, Loganathan Karthik, Man Wang, Ke-Feng Wang, Zhong Wang, Jing Yu, Yuting Shuai, Jiaming Yu, Lu Zhang, Zhiheng Yang, Chuan Li, Qian Zhang, Tong Shi, Liming Zhou, Feng Xie, Huanqin Dai, Xueting Liu, Jingyu Zhang, Guang Liu, Ying Zhuo, Buchang Zhang, Chenli Liu, Shanshan Li, Xuekui Xia, Yaojun Tong, Yanwen Liu, Gil Alterovitz, Gao-Yi Tan () and Li-Xin Zhang ()
Additional contact information
Mindong Liang: East China University of Science and Technology (ECUST)
Zilong Li: Chinese Academy of Sciences
Weishan Wang: Chinese Academy of Sciences
Jiakun Liu: East China University of Science and Technology (ECUST)
Leshi Liu: East China University of Science and Technology (ECUST)
Guoliang Zhu: East China University of Science and Technology (ECUST)
Loganathan Karthik: East China University of Science and Technology (ECUST)
Man Wang: Shanghai Jiao Tong University
Ke-Feng Wang: East China University of Science and Technology (ECUST)
Zhong Wang: Sixth People’s Hospital South Campus Affiliated to Shanghai Jiao Tong University
Jing Yu: Sixth People’s Hospital South Campus Affiliated to Shanghai Jiao Tong University
Yuting Shuai: East China University of Science and Technology (ECUST)
Jiaming Yu: East China University of Science and Technology (ECUST)
Lu Zhang: East China University of Science and Technology (ECUST)
Zhiheng Yang: East China University of Science and Technology (ECUST)
Chuan Li: East China University of Science and Technology (ECUST)
Qian Zhang: East China University of Science and Technology (ECUST)
Tong Shi: East China University of Science and Technology (ECUST)
Liming Zhou: East China University of Science and Technology (ECUST)
Feng Xie: Chinese Academy of Sciences
Huanqin Dai: Chinese Academy of Sciences
Xueting Liu: East China University of Science and Technology (ECUST)
Jingyu Zhang: East China University of Science and Technology (ECUST)
Guang Liu: East China University of Science and Technology (ECUST)
Ying Zhuo: East China University of Science and Technology (ECUST)
Buchang Zhang: Anhui University
Chenli Liu: Chinese Academy of Sciences
Shanshan Li: Chinese Academy of Agricultural Sciences
Xuekui Xia: Qilu University of Technology (Shandong Academy of Sciences)
Yaojun Tong: Technical University of Denmark
Yanwen Liu: inBiome Sciences
Gil Alterovitz: Harvard Medical School Countway Library
Gao-Yi Tan: East China University of Science and Technology (ECUST)
Li-Xin Zhang: East China University of Science and Technology (ECUST)

Nature Communications, 2019, vol. 10, issue 1, 1-9

Abstract: Abstract Besides genome editing, CRISPR-Cas12a has recently been used for DNA detection applications with attomolar sensitivity but, to our knowledge, it has not been used for the detection of small molecules. Bacterial allosteric transcription factors (aTFs) have evolved to sense and respond sensitively to a variety of small molecules to benefit bacterial survival. By combining the single-stranded DNA cleavage ability of CRISPR-Cas12a and the competitive binding activities of aTFs for small molecules and double-stranded DNA, here we develop a simple, supersensitive, fast and high-throughput platform for the detection of small molecules, designated CaT-SMelor (CRISPR-Cas12a- and aTF-mediated small molecule detector). CaT-SMelor is successfully evaluated by detecting nanomolar levels of various small molecules, including uric acid and p-hydroxybenzoic acid among their structurally similar analogues. We also demonstrate that our CaT-SMelor directly measured the uric acid concentration in clinical human blood samples, indicating a great potential of CaT-SMelor in the detection of small molecules.

Date: 2019
References: Add references at CitEc
Citations: View citations in EconPapers (1)

Downloads: (external link)
https://www.nature.com/articles/s41467-019-11648-1 Abstract (text/html)

Related works:
This item may be available elsewhere in EconPapers: Search for items with the same title.

Export reference: BibTeX RIS (EndNote, ProCite, RefMan) HTML/Text

Persistent link: https://EconPapers.repec.org/RePEc:nat:natcom:v:10:y:2019:i:1:d:10.1038_s41467-019-11648-1

Ordering information: This journal article can be ordered from
https://www.nature.com/ncomms/

DOI: 10.1038/s41467-019-11648-1

Access Statistics for this article

Nature Communications is currently edited by Nathalie Le Bot, Enda Bergin and Fiona Gillespie

More articles in Nature Communications from Nature
Bibliographic data for series maintained by Sonal Shukla () and Springer Nature Abstracting and Indexing ().