Heterogeneity of human bone marrow and blood natural killer cells defined by single-cell transcriptome

Chao Yang, Jason R. Siebert, Robert Burns, Zachary J. Gerbec, Benedetta Bonacci, Amy Rymaszewski, Mary Rau, Matthew J. Riese, Sridhar Rao, Karen-Sue Carlson, John M. Routes, James W. Verbsky, Monica S. Thakar and Subramaniam Malarkannan ()
Additional contact information
Chao Yang: Blood Research Institute, Versiti
Jason R. Siebert: Blood Research Institute, Versiti
Robert Burns: Blood Research Institute, Versiti
Zachary J. Gerbec: Blood Research Institute, Versiti
Benedetta Bonacci: Blood Research Institute, Versiti
Amy Rymaszewski: Medical College of Wisconsin
Mary Rau: Medical College of Wisconsin
Matthew J. Riese: Medical College of Wisconsin
Sridhar Rao: Medical College of Wisconsin
Karen-Sue Carlson: Medical College of Wisconsin
John M. Routes: Medical College of Wisconsin
James W. Verbsky: Medical College of Wisconsin
Monica S. Thakar: Blood Research Institute, Versiti
Subramaniam Malarkannan: Blood Research Institute, Versiti

Nature Communications, 2019, vol. 10, issue 1, 1-16

Abstract: Abstract Natural killer (NK) cells are critical to both innate and adaptive immunity. However, the development and heterogeneity of human NK cells are yet to be fully defined. Using single-cell RNA-sequencing technology, here we identify distinct NK populations in human bone marrow and blood, including one population expressing higher levels of immediate early genes indicative of a homeostatic activation. Functionally matured NK cells with high expression of CX3CR1, HAVCR2 (TIM-3), and ZEB2 represents terminally differentiated status with the unique transcriptional profile. Transcriptomic and pseudotime analyses identify a transitional population between CD56bright and CD56dim NK cells. Finally, a donor with GATA2T354M mutation exhibits reduced percentage of CD56bright NK cells with altered transcriptome and elevated cell death. These data expand our understanding of the heterogeneity and development of human NK cells.

Date: 2019
References: Add references at CitEc
Citations: View citations in EconPapers (7)

Downloads: (external link)
https://www.nature.com/articles/s41467-019-11947-7 Abstract (text/html)

Related works:
This item may be available elsewhere in EconPapers: Search for items with the same title.

Export reference: BibTeX RIS (EndNote, ProCite, RefMan) HTML/Text

Persistent link: https://EconPapers.repec.org/RePEc:nat:natcom:v:10:y:2019:i:1:d:10.1038_s41467-019-11947-7

Ordering information: This journal article can be ordered from
https://www.nature.com/ncomms/

DOI: 10.1038/s41467-019-11947-7

Access Statistics for this article

Nature Communications is currently edited by Nathalie Le Bot, Enda Bergin and Fiona Gillespie

More articles in Nature Communications from Nature
Bibliographic data for series maintained by Sonal Shukla () and Springer Nature Abstracting and Indexing ().