Misshapen coordinates protrusion restriction and actomyosin contractility during collective cell migration

Cédric Plutoni, Sarah Keil, Carlos Zeledon, Lara Elis Alberici Delsin, Barbara Decelle, Philippe P. Roux, Sébastien Carréno and Gregory Emery ()
Additional contact information
Cédric Plutoni: Université de Montréal
Sarah Keil: Université de Montréal
Carlos Zeledon: Université de Montréal
Lara Elis Alberici Delsin: Université de Montréal
Barbara Decelle: Université de Montréal
Philippe P. Roux: Université de Montréal
Sébastien Carréno: Université de Montréal
Gregory Emery: Université de Montréal

Nature Communications, 2019, vol. 10, issue 1, 1-16

Abstract: Abstract Collective cell migration is involved in development, wound healing and metastasis. In the Drosophila ovary, border cells (BC) form a small cluster that migrates collectively through the egg chamber. To achieve directed motility, the BC cluster coordinates the formation of protrusions in its leader cell and contractility at the rear. Restricting protrusions to leader cells requires the actin and plasma membrane linker Moesin. Herein, we show that the Ste20-like kinase Misshapen phosphorylates Moesin in vitro and in BC. Depletion of Misshapen disrupts protrusion restriction, thereby allowing other cells within the cluster to protrude. In addition, we show that Misshapen is critical to generate contractile forces both at the rear of the cluster and at the base of protrusions. Together, our results indicate that Misshapen is a key regulator of BC migration as it coordinates two independent pathways that restrict protrusion formation to the leader cells and induces contractile forces.

Date: 2019
References: Add references at CitEc
Citations: View citations in EconPapers (1)

Downloads: (external link)
https://www.nature.com/articles/s41467-019-11963-7 Abstract (text/html)

Related works:
This item may be available elsewhere in EconPapers: Search for items with the same title.

Export reference: BibTeX RIS (EndNote, ProCite, RefMan) HTML/Text

Persistent link: https://EconPapers.repec.org/RePEc:nat:natcom:v:10:y:2019:i:1:d:10.1038_s41467-019-11963-7

Ordering information: This journal article can be ordered from
https://www.nature.com/ncomms/

DOI: 10.1038/s41467-019-11963-7

Access Statistics for this article

Nature Communications is currently edited by Nathalie Le Bot, Enda Bergin and Fiona Gillespie

More articles in Nature Communications from Nature
Bibliographic data for series maintained by Sonal Shukla () and Springer Nature Abstracting and Indexing ().