In vitro role of Rad54 in Rad51-ssDNA filament-dependent homology search and synaptic complexes formation

Eliana Moreira Tavares, William Douglass Wright, Wolf-Dietrich Heyer, Eric Le Cam and Pauline Dupaigne ()
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Eliana Moreira Tavares: Genome Maintenance and Molecular Microscopy UMR8126 CNRS, Université Paris-Sud, Université Paris-Saclay, Gustave Roussy
William Douglass Wright: University of California, Davis
Wolf-Dietrich Heyer: University of California, Davis
Eric Le Cam: Genome Maintenance and Molecular Microscopy UMR8126 CNRS, Université Paris-Sud, Université Paris-Saclay, Gustave Roussy
Pauline Dupaigne: Genome Maintenance and Molecular Microscopy UMR8126 CNRS, Université Paris-Sud, Université Paris-Saclay, Gustave Roussy

Nature Communications, 2019, vol. 10, issue 1, 1-12

Abstract: Abstract Homologous recombination (HR) uses a homologous template to accurately repair DNA double-strand breaks and stalled replication forks to maintain genome stability. During homology search, Rad51 nucleoprotein filaments probe and interact with dsDNA, forming the synaptic complex that is stabilized on a homologous sequence. Strand intertwining leads to the formation of a displacement-loop (D-loop). In yeast, Rad54 is essential for HR in vivo and required for D-loop formation in vitro, but its exact role remains to be fully elucidated. Using electron microscopy to visualize the DNA-protein complexes, here we find that Rad54 is crucial for Rad51-mediated synaptic complex formation and homology search. The Rad54−K341R ATPase-deficient mutant protein promotes formation of synaptic complexes but not D-loops and leads to the accumulation of stable heterologous associations, suggesting that the Rad54 ATPase is involved in preventing non-productive intermediates. We propose that Rad51/Rad54 form a functional unit operating in homology search, synaptic complex and D-loop formation.

Date: 2019
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DOI: 10.1038/s41467-019-12082-z

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