Ran pathway-independent regulation of mitotic Golgi disassembly by Importin-α

Chih-Chia Chang, Ching-Jou Chen, Cédric Grauffel, Yu-Chung Pien, Carmay Lim, Su-Yi Tsai () and Kuo-Chiang Hsia ()
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Chih-Chia Chang: Academia Sinica
Ching-Jou Chen: National Taiwan University
Cédric Grauffel: Academia Sinica
Yu-Chung Pien: National Taiwan University
Carmay Lim: Academia Sinica
Su-Yi Tsai: National Taiwan University
Kuo-Chiang Hsia: Academia Sinica

Nature Communications, 2019, vol. 10, issue 1, 1-16

Abstract: Abstract To facilitate proper mitotic cell partitioning, the Golgi disassembles by suppressing vesicle fusion. However, the underlying mechanism has not been characterized previously. Here, we report a Ran pathway-independent attenuation mechanism that allows Importin-α (a nuclear transport factor) to suppress the vesicle fusion mediated by p115 (a vesicular tethering factor) and is required for mitotic Golgi disassembly. We demonstrate that Importin-α directly competes with p115 for interaction with the Golgi protein GM130. This interaction, promoted by a phosphate moiety on GM130, is independent of Importin-β and Ran. A GM130 K34A mutant, in which the Importin-α-GM130 interaction is specifically disrupted, exhibited abundant Golgi puncta during metaphase. Importantly, a mutant showing enhanced p115-GM130 interaction presented proliferative defects and G2/M arrest, demonstrating that Importin-α-GM130 binding modulates the Golgi disassembly that governs mitotic progression. Our findings illuminate that the Ran and kinase-phosphatase pathways regulate multiple aspects of mitosis coordinated by Importin-α (e.g. spindle assembly, Golgi disassembly).

Date: 2019
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DOI: 10.1038/s41467-019-12207-4

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