A tunable dual-input system for on-demand dynamic gene expression regulation

Elisa Pedone (), Lorena Postiglione, Francesco Aulicino, Dan L. Rocca (), Sandra Montes-Olivas, Mahmoud Khazim, Diego Bernardo, Maria Pia Cosma and Lucia Marucci ()
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Elisa Pedone: University of Bristol
Lorena Postiglione: University of Bristol
Francesco Aulicino: BrisSynBio
Dan L. Rocca: University of Bristol
Sandra Montes-Olivas: University of Bristol
Mahmoud Khazim: University of Bristol
Diego Bernardo: Telethon Institute of Genetics and Medicine Via Campi Flegrei 34
Maria Pia Cosma: Centre for Genomic Regulation (CRG)
Lucia Marucci: University of Bristol

Nature Communications, 2019, vol. 10, issue 1, 1-13

Abstract: Abstract Cellular systems have evolved numerous mechanisms to adapt to environmental stimuli, underpinned by dynamic patterns of gene expression. In addition to gene transcription regulation, modulation of protein levels, dynamics and localization are essential checkpoints governing cell functions. The introduction of inducible promoters has allowed gene expression control using orthogonal molecules, facilitating its rapid and reversible manipulation to study gene function. However, differing protein stabilities hinder the generation of protein temporal profiles seen in vivo. Here, we improve the Tet-On system integrating conditional destabilising elements at the post-translational level and permitting simultaneous control of gene expression and protein stability. We show, in mammalian cells, that adding protein stability control allows faster response times, fully tunable and enhanced dynamic range, and improved in silico feedback control of gene expression. Finally, we highlight the effectiveness of our dual-input system to modulate levels of signalling pathway components in mouse Embryonic Stem Cells.

Date: 2019
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DOI: 10.1038/s41467-019-12329-9

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