FIERY1 promotes microRNA accumulation by suppressing rRNA-derived small interfering RNAs in Arabidopsis

Chenjiang You, Wenrong He, Runlai Hang, Cuiju Zhang, Xiaofeng Cao, Hongwei Guo, Xuemei Chen, Jie Cui () and Beixin Mo ()
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Chenjiang You: Shenzhen University
Wenrong He: University of California
Runlai Hang: Shenzhen University
Cuiju Zhang: Shenzhen University
Xiaofeng Cao: Chinese Academy of Sciences
Hongwei Guo: Southern University of Science and Technology of China
Xuemei Chen: University of California
Jie Cui: Shenzhen University
Beixin Mo: Shenzhen University

Nature Communications, 2019, vol. 10, issue 1, 1-15

Abstract: Abstract Plant microRNAs (miRNAs) associate with ARGONAUTE1 (AGO1) to direct post-transcriptional gene silencing and regulate numerous biological processes. Although AGO1 predominantly binds miRNAs in vivo, it also associates with endogenous small interfering RNAs (siRNAs). It is unclear whether the miRNA/siRNA balance affects miRNA activities. Here we report that FIERY1 (FRY1), which is involved in 5′−3′ RNA degradation, regulates miRNA abundance and function by suppressing the biogenesis of ribosomal RNA-derived siRNAs (risiRNAs). In mutants of FRY1 and the nuclear 5′−3′ exonuclease genes XRN2 and XRN3, we find that a large number of 21-nt risiRNAs are generated through an endogenous siRNA biogenesis pathway. The production of risiRNAs correlates with pre-rRNA processing defects in these mutants. We also show that these risiRNAs are loaded into AGO1, causing reduced loading of miRNAs. This study reveals a previously unknown link between rRNA processing and miRNA accumulation.

Date: 2019
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DOI: 10.1038/s41467-019-12379-z

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