CENP-A nucleosome clusters form rosette-like structures around HJURP during G1

Leonid Andronov, Khalid Ouararhni, Isabelle Stoll, Bruno P. Klaholz () and Ali Hamiche ()
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Leonid Andronov: Université de Strasbourg
Khalid Ouararhni: Institute of Genetics and of Molecular and Cellular Biology (IGBMC)
Isabelle Stoll: Institute of Genetics and of Molecular and Cellular Biology (IGBMC)
Bruno P. Klaholz: Université de Strasbourg
Ali Hamiche: Institute of Genetics and of Molecular and Cellular Biology (IGBMC)

Nature Communications, 2019, vol. 10, issue 1, 1-8

Abstract: Abstract CENP-A is an essential histone H3 variant that epigenetically marks the centromeric region of chromosomes. Here we show that CENP-A nucleosomes form characteristic clusters during the G1 phase of the cell cycle. 2D and 3D super-resolution microscopy and segmentation analysis reveal that these clusters encompass a globular rosette-like structure, which evolves into a more compact structure in late G1. The rosette-like clusters contain numerous CENP-A molecules and form a large cellular structure of ∼250–300 nm diameter with remarkably similar shapes for each centromere. Co-localization analysis shows that HJURP, the CENP-A chaperone, is located in the center of the rosette and serves as a nucleation point. The discovery of an HJURP-mediated CENP-A nucleation in human cells and its structural description provide important insights into the mechanism of CENP-A deposition and the organization of CENP-A chromatin in the centromeric region.

Date: 2019
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DOI: 10.1038/s41467-019-12383-3

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