Photoactivation of silicon rhodamines via a light-induced protonation

Michelle S. Frei, Philipp Hoess, Marko Lampe, Bianca Nijmeijer, Moritz Kueblbeck, Jan Ellenberg, Hubert Wadepohl, Jonas Ries, Stefan Pitsch, Luc Reymond () and Kai Johnsson ()
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Michelle S. Frei: Max Planck Institute for Medical Research
Philipp Hoess: European Molecular Biology Laboratory (EMBL)
Marko Lampe: European Molecular Biology Laboratory (EMBL)
Bianca Nijmeijer: European Molecular Biology Laboratory (EMBL)
Moritz Kueblbeck: European Molecular Biology Laboratory (EMBL)
Jan Ellenberg: European Molecular Biology Laboratory (EMBL)
Hubert Wadepohl: University of Heidelberg
Jonas Ries: European Molecular Biology Laboratory (EMBL)
Stefan Pitsch: Spirochrome AG
Luc Reymond: École Polytechnique Fédérale de Lausanne (EPFL)
Kai Johnsson: Max Planck Institute for Medical Research

Nature Communications, 2019, vol. 10, issue 1, 1-10

Abstract: Abstract Photoactivatable fluorophores are important for single-particle tracking and super-resolution microscopy. Here we present a photoactivatable fluorophore that forms a bright silicon rhodamine derivative through a light-dependent protonation. In contrast to other photoactivatable fluorophores, no caging groups are required, nor are there any undesired side-products released. Using this photoactivatable fluorophore, we create probes for HaloTag and actin for live-cell single-molecule localization microscopy and single-particle tracking experiments. The unusual mechanism of photoactivation and the fluorophore’s outstanding spectroscopic properties make it a powerful tool for live-cell super-resolution microscopy.

Date: 2019
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DOI: 10.1038/s41467-019-12480-3

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