Occurrence and repair of alkylating stress in the intracellular pathogen Brucella abortus

Katy Poncin, Agnès Roba, Ravikumar Jimmidi, Georges Potemberg, Antonella Fioravanti, Nayla Francis, Kévin Willemart, Nicolas Zeippen, Arnaud Machelart, Emanuele G. Biondi, Eric Muraille, Stéphane P. Vincent and Xavier De Bolle ()
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Katy Poncin: URBM, Narilis, University of Namur
Agnès Roba: URBM, Narilis, University of Namur
Ravikumar Jimmidi: University of Namur
Georges Potemberg: URBM, Narilis, University of Namur
Antonella Fioravanti: UMR 8576 CNRS, Université de Lille
Nayla Francis: URBM, Narilis, University of Namur
Kévin Willemart: URBM, Narilis, University of Namur
Nicolas Zeippen: URBM, Narilis, University of Namur
Arnaud Machelart: URBM, Narilis, University of Namur
Emanuele G. Biondi: UMR 8576 CNRS, Université de Lille
Eric Muraille: IMM, 31 Chemin Joseph Aiguier, 13009 Marseille, Aix-Marseille Université
Stéphane P. Vincent: University of Oxford
Xavier De Bolle: URBM, Narilis, University of Namur

Nature Communications, 2019, vol. 10, issue 1, 1-13

Abstract: Abstract It is assumed that intracellular pathogenic bacteria have to cope with DNA alkylating stress within host cells. Here we use single-cell reporter systems to show that the pathogen Brucella abortus does encounter alkylating stress during the first hours of macrophage infection. Genes encoding direct repair and base-excision repair pathways are required by B. abortus to face this stress in vitro and in a mouse infection model. Among these genes, ogt is found to be under the control of the conserved cell-cycle transcription factor GcrA. Our results highlight that the control of DNA repair in B. abortus displays distinct features that are not present in model organisms such as Escherichia coli.

Date: 2019
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DOI: 10.1038/s41467-019-12516-8

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