Meiotic crossovers characterized by haplotype-specific chromosome painting in maize

Lívia Martins, Fan Yu, Hainan Zhao, Tesia Dennison, Nick Lauter, Haiyan Wang, Zuhu Deng, Addie Thompson, Kassandra Semrau, Jean-Marie Rouillard, James A. Birchler and Jiming Jiang ()
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Lívia Martins: Michigan State University
Fan Yu: Michigan State University
Hainan Zhao: Michigan State University
Tesia Dennison: Iowa State University
Nick Lauter: Iowa State University
Haiyan Wang: Michigan State University
Zuhu Deng: Fujian Agriculture and Forestry University
Addie Thompson: Michigan State University
Kassandra Semrau: Arbor Biosciences
Jean-Marie Rouillard: Arbor Biosciences
James A. Birchler: University of Missouri
Jiming Jiang: Michigan State University

Nature Communications, 2019, vol. 10, issue 1, 1-10

Abstract: Abstract Meiotic crossovers (COs) play a critical role in generating genetic variation and maintaining faithful segregation of homologous chromosomes during meiosis. We develop a haplotype-specific fluorescence in situ hybridization (FISH) technique that allows visualization of COs directly on metaphase chromosomes. Oligonucleotides (oligos) specific to chromosome 10 of maize inbreds B73 and Mo17, respectively, are synthesized and labeled as FISH probes. The parental and recombinant chromosome 10 in B73 x Mo17 F1 hybrids and F2 progenies can be unambiguously identified by haplotype-specific FISH. Analysis of 58 F2 plants reveals lack of COs in the entire proximal half of chromosome 10. However, we detect COs located in regions very close to the centromere in recombinant inbred lines from an intermated B73 x Mo17 population, suggesting effective accumulation of COs in recombination-suppressed chromosomal regions through intermating and the potential to generate favorable allelic combinations of genes residing in these regions.

Date: 2019
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DOI: 10.1038/s41467-019-12646-z

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