Plant AtEH/Pan1 proteins drive autophagosome formation at ER-PM contact sites with actin and endocytic machinery

Pengwei Wang, Roman Pleskot, Jingze Zang, Joanna Winkler, Jie Wang, Klaas Yperman, Tong Zhang, Kun Wang, Jinli Gong, Yajie Guan, Christine Richardson, Patrick Duckney, Michael Vandorpe, Evelien Mylle, Jindriska Fiserova, Daniel Van Damme () and Patrick J. Hussey ()
Additional contact information
Pengwei Wang: Durham University
Roman Pleskot: Ghent University
Jingze Zang: Durham University
Joanna Winkler: Ghent University
Jie Wang: Ghent University
Klaas Yperman: Ghent University
Tong Zhang: Huazhong Agricultural University
Kun Wang: Huazhong Agricultural University
Jinli Gong: Huazhong Agricultural University
Yajie Guan: Huazhong Agricultural University
Christine Richardson: Durham University
Patrick Duckney: Durham University
Michael Vandorpe: Ghent University
Evelien Mylle: Ghent University
Jindriska Fiserova: Durham University
Daniel Van Damme: Ghent University
Patrick J. Hussey: Durham University

Nature Communications, 2019, vol. 10, issue 1, 1-16

Abstract: Abstract The Arabidopsis EH proteins (AtEH1/Pan1 and AtEH2/Pan1) are components of the endocytic TPLATE complex (TPC) which is essential for endocytosis. Both proteins are homologues of the yeast ARP2/3 complex activator, Pan1p. Here, we show that these proteins are also involved in actin cytoskeleton regulated autophagy. Both AtEH/Pan1 proteins localise to the plasma membrane and autophagosomes. Upon induction of autophagy, AtEH/Pan1 proteins recruit TPC and AP-2 subunits, clathrin, actin and ARP2/3 proteins to autophagosomes. Increased expression of AtEH/Pan1 proteins boosts autophagosome formation, suggesting independent and redundant pathways for actin-mediated autophagy in plants. Moreover, AtEHs/Pan1-regulated autophagosomes associate with ER-PM contact sites (EPCS) where AtEH1/Pan1 interacts with VAP27-1. Knock-down expression of either AtEH1/Pan1 or VAP27-1 makes plants more susceptible to nutrient depleted conditions, indicating that the autophagy pathway is perturbed. In conclusion, we identify the existence of an autophagy-dependent pathway in plants to degrade endocytic components, starting at the EPCS through the interaction among AtEH/Pan1, actin cytoskeleton and the EPCS resident protein VAP27-1.

Date: 2019
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DOI: 10.1038/s41467-019-12782-6

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