Adaptive prospective optical gating enables day-long 3D time-lapse imaging of the beating embryonic zebrafish heart

Jonathan M. Taylor (), Carl J. Nelson, Finnius A. Bruton, Aryan Kaveh, Charlotte Buckley, Carl S. Tucker, Adriano G. Rossi, John J. Mullins and Martin A. Denvir
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Jonathan M. Taylor: University of Glasgow
Carl J. Nelson: University of Glasgow
Finnius A. Bruton: Queen’s Medical Research Institute, University of Edinburgh
Aryan Kaveh: Queen’s Medical Research Institute, University of Edinburgh
Charlotte Buckley: Queen’s Medical Research Institute, University of Edinburgh
Carl S. Tucker: Queen’s Medical Research Institute, University of Edinburgh
Adriano G. Rossi: University of Edinburgh Medical School
John J. Mullins: Queen’s Medical Research Institute, University of Edinburgh
Martin A. Denvir: Queen’s Medical Research Institute, University of Edinburgh

Nature Communications, 2019, vol. 10, issue 1, 1-15

Abstract: Abstract Three-dimensional fluorescence time-lapse imaging of the beating heart is extremely challenging, due to the heart’s constant motion and a need to avoid pharmacological or phototoxic damage. Although real-time triggered imaging can computationally “freeze” the heart for 3D imaging, no previous algorithm has been able to maintain phase-lock across developmental timescales. We report a new algorithm capable of maintaining day-long phase-lock, permitting routine acquisition of synchronised 3D + time video time-lapse datasets of the beating zebrafish heart. This approach has enabled us for the first time to directly observe detailed developmental and cellular processes in the beating heart, revealing the dynamics of the immune response to injury and witnessing intriguing proliferative events that challenge the established literature on cardiac trabeculation. Our approach opens up exciting new opportunities for direct time-lapse imaging studies over a 24-hour time course, to understand the cellular mechanisms underlying cardiac development, repair and regeneration.

Date: 2019
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DOI: 10.1038/s41467-019-13112-6

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