Packaging and delivering enzymes by amorphous metal-organic frameworks

Xiaoling Wu, Hua Yue, Yuanyu Zhang, Xiaoyong Gao, Xiaoyang Li, Licheng Wang, Yufei Cao, Miao Hou, Haixia An, Lin Zhang (), Sai Li (), Jingyuan Ma, He Lin, Yanan Fu, Hongkai Gu, Wenyong Lou, Wei Wei (), Richard N. Zare and Jun Ge ()
Additional contact information
Xiaoling Wu: Tsinghua University
Hua Yue: Chinese Academy of Sciences
Yuanyu Zhang: Tsinghua University
Xiaoyong Gao: Chinese Academy of Sciences
Xiaoyang Li: Tsinghua University
Licheng Wang: Tsinghua University
Yufei Cao: Tsinghua University
Miao Hou: Tsinghua University
Haixia An: Chinese Academy of Sciences
Lin Zhang: Tianjin University
Sai Li: Tsinghua University
Jingyuan Ma: Chinese Academy of Sciences
He Lin: Chinese Academy of Sciences
Yanan Fu: Chinese Academy of Sciences
Hongkai Gu: Jilin University
Wenyong Lou: South China University of Technology
Wei Wei: Chinese Academy of Sciences
Richard N. Zare: Fudan University, Jiangwan Campus
Jun Ge: Tsinghua University

Nature Communications, 2019, vol. 10, issue 1, 1-8

Abstract: Abstract Enzymatic catalysis in living cells enables the in-situ detection of cellular metabolites in single cells, which could contribute to early diagnosis of diseases. In this study, enzyme is packaged in amorphous metal-organic frameworks (MOFs) via a one-pot co-precipitation process under ambient conditions, exhibiting 5–20 times higher apparent activity than when the enzyme is encapsulated in corresponding crystalline MOFs. Molecular simulation and cryo-electron tomography (Cryo-ET) combined with other techniques demonstrate that the mesopores generated in this disordered and fuzzy structure endow the packaged enzyme with high enzyme activity. The highly active glucose oxidase delivered by the amorphous MOF nanoparticles allows the noninvasive and facile measurement of glucose in single living cells, which can be used to distinguish between cancerous and normal cells.

Date: 2019
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DOI: 10.1038/s41467-019-13153-x

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