SERS discrimination of single DNA bases in single oligonucleotides by electro-plasmonic trapping

Jian-An Huang, Mansoureh Z. Mousavi, Yingqi Zhao, Aliaksandr Hubarevich, Fatima Omeis, Giorgia Giovannini, Moritz Schütte, Denis Garoli () and Francesco De Angelis ()
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Jian-An Huang: Istituto Italiano di Tecnologia
Mansoureh Z. Mousavi: Istituto Italiano di Tecnologia
Yingqi Zhao: Istituto Italiano di Tecnologia
Aliaksandr Hubarevich: Istituto Italiano di Tecnologia
Fatima Omeis: Istituto Italiano di Tecnologia
Giorgia Giovannini: Istituto Italiano di Tecnologia
Moritz Schütte: Alacris Theranostics GmbH
Denis Garoli: Istituto Italiano di Tecnologia
Francesco De Angelis: Istituto Italiano di Tecnologia

Nature Communications, 2019, vol. 10, issue 1, 1-10

Abstract: Abstract Surface-enhanced Raman spectroscopy (SERS) sensing of DNA bases by plasmonic nanopores could pave a way to novel methods for DNA analyses and new generation single-molecule sequencing platforms. The SERS discrimination of single DNA bases depends critically on the time that a DNA strand resides within the plasmonic hot spot. In fact, DNA molecules flow through the nanopores so rapidly that the SERS signals collected are not sufficient for single-molecule analysis. Here, we report an approach to control the residence time of molecules in the hot spot by an electro-plasmonic trapping effect. By directly adsorbing molecules onto a gold nanoparticle and then trapping the single nanoparticle in a plasmonic nanohole up to several minutes, we demonstrate single-molecule SERS detection of all four DNA bases as well as discrimination of single nucleobases in a single oligonucleotide. Our method can be extended easily to label-free sensing of single-molecule amino acids and proteins.

Date: 2019
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DOI: 10.1038/s41467-019-13242-x

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