Identification of the periplasmic DNA receptor for natural transformation of Helicobacter pylori

Prashant P. Damke, Anne Marie Di Guilmi, Paloma Fernández Varela, Christophe Velours, Stéphanie Marsin, Xavier Veaute, Mérick Machouri, Gaurav V. Gunjal, Desirazu N. Rao, Jean-Baptiste Charbonnier and J. Pablo Radicella ()
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Prashant P. Damke: CEA, Université de Paris and Université Paris Sud
Anne Marie Di Guilmi: CEA, Université de Paris and Université Paris Sud
Paloma Fernández Varela: CEA, CNRS, Univ. Paris‐Sud, Université Paris‐Saclay
Christophe Velours: CEA, CNRS, Univ. Paris‐Sud, Université Paris‐Saclay
Stéphanie Marsin: CEA, CNRS, Univ. Paris‐Sud, Université Paris‐Saclay
Xavier Veaute: Universités Paris Diderot and Paris Sud
Mérick Machouri: CEA, Université de Paris and Université Paris Sud
Gaurav V. Gunjal: Indian Institute of Science
Desirazu N. Rao: Indian Institute of Science
Jean-Baptiste Charbonnier: CEA, CNRS, Univ. Paris‐Sud, Université Paris‐Saclay
J. Pablo Radicella: CEA, Université de Paris and Université Paris Sud

Nature Communications, 2019, vol. 10, issue 1, 1-11

Abstract: Abstract Horizontal gene transfer through natural transformation is a major driver of antibiotic resistance spreading in many pathogenic bacterial species. In the case of Gram-negative bacteria, and in particular of Helicobacter pylori, the mechanisms underlying the handling of the incoming DNA within the periplasm are poorly understood. Here we identify the protein ComH as the periplasmic receptor for the transforming DNA during natural transformation in H. pylori. ComH is a DNA-binding protein required for the import of DNA into the periplasm. Its C-terminal domain displays strong affinity for double-stranded DNA and is sufficient for the accumulation of DNA in the periplasm, but not for DNA internalisation into the cytoplasm. The N-terminal region of the protein allows the interaction of ComH with a periplasmic domain of the inner-membrane channel ComEC, which is known to mediate the translocation of DNA into the cytoplasm. Our results indicate that ComH is involved in the import of DNA into the periplasm and its delivery to the inner membrane translocator ComEC.

Date: 2019
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DOI: 10.1038/s41467-019-13352-6

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