Classification of mouse B cell types using surfaceome proteotype maps

Marc Oostrum, Maik Müller, Fabian Klein, Roland Bruderer, Hui Zhang, Patrick G. A. Pedrioli, Lukas Reiter, Panagiotis Tsapogas, Antonius Rolink and Bernd Wollscheid ()
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Marc Oostrum: ETH Zurich
Maik Müller: ETH Zurich
Fabian Klein: University of Basel
Roland Bruderer: Biognosys AG
Hui Zhang: Johns Hopkins University
Patrick G. A. Pedrioli: ETH Zurich
Lukas Reiter: Biognosys AG
Panagiotis Tsapogas: University of Basel
Antonius Rolink: University of Basel
Bernd Wollscheid: ETH Zurich

Nature Communications, 2019, vol. 10, issue 1, 1-9

Abstract: Abstract System-wide quantification of the cell surface proteotype and identification of extracellular glycosylation sites is challenging when samples are limited. Here, we miniaturize and automate the previously described Cell Surface Capture (CSC) technology, increasing sensitivity, reproducibility and throughput. We use this technology, which we call autoCSC, to create population-specific surfaceome maps of developing mouse B cells and use targeted flow cytometry to uncover developmental cell subpopulations.

Date: 2019
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DOI: 10.1038/s41467-019-13418-5

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