Photo-activated raster scanning thermal imaging at sub-diffraction resolution

M. Bouzin, M. Marini, A. Zeynali, M. Borzenkov, L. Sironi, L. D’Alfonso, F. Mingozzi, F. Granucci, P. Pallavicini, G. Chirico () and M. Collini
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M. Bouzin: Università degli Studi di Milano-Bicocca
M. Marini: Università degli Studi di Milano-Bicocca
A. Zeynali: Università degli Studi di Milano-Bicocca
M. Borzenkov: Università degli Studi di Milano-Bicocca
L. Sironi: Università degli Studi di Milano-Bicocca
L. D’Alfonso: Università degli Studi di Milano-Bicocca
F. Mingozzi: Università degli Studi di Milano-Bicocca
F. Granucci: Università degli Studi di Milano-Bicocca
P. Pallavicini: Università degli Studi di Pavia
G. Chirico: Università degli Studi di Milano-Bicocca
M. Collini: Università degli Studi di Milano-Bicocca

Nature Communications, 2019, vol. 10, issue 1, 1-9

Abstract: Abstract Active thermal imaging is a valuable tool for the nondestructive characterization of the morphological properties and the functional state of biological tissues and synthetic materials. However, state-of-the-art techniques do not typically combine the required high spatial resolution over extended fields of view with the quantification of temperature variations. Here, we demonstrate quantitative far-infrared photo-thermal imaging at sub-diffraction resolution over millimeter-sized fields of view. Our approach combines the sample absorption of modulated raster-scanned laser light with the automated localization of the laser-induced temperature variations imaged by a thermal camera. With temperature increments ∼0.5–5 °C, we achieve a six-time gain with respect to our 350-μm diffraction-limited resolution with proof-of-principle experiments on synthetic samples. We finally demonstrate the biological relevance of sub-diffraction thermal imaging by retrieving temperature-based super-resolution maps of the distribution of Prussian blue nanocubes across explanted murine skin biopsies.

Date: 2019
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DOI: 10.1038/s41467-019-13447-0

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