Multi-scale imaging and analysis identify pan-embryo cell dynamics of germlayer formation in zebrafish

Gopi Shah (), Konstantin Thierbach, Benjamin Schmid, Johannes Waschke, Anna Reade, Mario Hlawitschka, Ingo Roeder, Nico Scherf () and Jan Huisken ()
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Gopi Shah: Max Planck Institute of Molecular Cell Biology and Genetics
Konstantin Thierbach: Carl Gustav Carus Faculty of Medicine, TU Dresden
Benjamin Schmid: Max Planck Institute of Molecular Cell Biology and Genetics
Johannes Waschke: Max Planck Institute for Human Cognitive and Brain Sciences
Anna Reade: University of California
Mario Hlawitschka: Leipzig University of Applied Sciences
Ingo Roeder: Carl Gustav Carus Faculty of Medicine, TU Dresden
Nico Scherf: Max Planck Institute of Molecular Cell Biology and Genetics
Jan Huisken: Max Planck Institute of Molecular Cell Biology and Genetics

Nature Communications, 2019, vol. 10, issue 1, 1-12

Abstract: Abstract The coordination of cell movements across spatio-temporal scales ensures precise positioning of organs during vertebrate gastrulation. Mechanisms governing such morphogenetic movements have been studied only within a local region, a single germlayer or in whole embryos without cell identity. Scale-bridging imaging and automated analysis of cell dynamics are needed for a deeper understanding of tissue formation during gastrulation. Here, we report pan-embryo analyses of formation and dynamics of all three germlayers simultaneously within a developing zebrafish embryo. We show that a distinct distribution of cells in each germlayer is established during early gastrulation via cell movement characteristics that are predominantly determined by their position in the embryo. The differences in initial germlayer distributions are subsequently amplified by a global movement, which organizes the organ precursors along the embryonic body axis, giving rise to the blueprint of organ formation. The tools and data are available as a resource for the community.

Date: 2019
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DOI: 10.1038/s41467-019-13625-0

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