Measuring local-directional resolution and local anisotropy in cryo-EM maps

Jose Luis Vilas, Hemant D. Tagare, Javier Vargas, Jose Maria Carazo () and Carlos Oscar S. Sorzano ()
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Jose Luis Vilas: Centro Nacional de Biotecnologia (CNB-CSIC)
Hemant D. Tagare: Yale University
Javier Vargas: McGill University
Jose Maria Carazo: Centro Nacional de Biotecnologia (CNB-CSIC)
Carlos Oscar S. Sorzano: Centro Nacional de Biotecnologia (CNB-CSIC)

Nature Communications, 2020, vol. 11, issue 1, 1-7

Abstract: Abstract The introduction of local resolution has enormously helped the understanding of cryo-EM maps. Still, for any given pixel it is a global, aggregated value, that makes impossible the individual analysis of the contribution of the different projection directions. We introduce MonoDir, a fully automatic, parameter-free method that, starting only from the final cryo-EM map, decomposes local resolution into the different projection directions, providing a detailed level of analysis of the final map. Many applications of directional local resolution are possible, and we concentrate here on map quality and validation.

Date: 2020
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DOI: 10.1038/s41467-019-13742-w

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