Conditional control of RNA-guided nucleic acid cleavage and gene editing

Shao-Ru Wang, Ling-Yu Wu, Hai-Yan Huang, Wei Xiong, Jian Liu, Lai Wei, Ping Yin, Tian Tian () and Xiang Zhou ()
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Shao-Ru Wang: Wuhan University
Ling-Yu Wu: Wuhan University
Hai-Yan Huang: Wuhan University
Wei Xiong: Wuhan University
Jian Liu: Huazhong Agricultural University
Lai Wei: Wuhan University
Ping Yin: Huazhong Agricultural University
Tian Tian: Wuhan University
Xiang Zhou: Wuhan University

Nature Communications, 2020, vol. 11, issue 1, 1-10

Abstract: Abstract Prokaryotes use repetitive genomic elements termed CRISPR (clustered regularly interspaced short palindromic repeats) to destroy invading genetic molecules. Although CRISPR systems have been widely used in DNA and RNA technology, certain adverse effects do occur. For example, constitutively active CRISPR systems may lead to a certain risk of off-target effects. Here, we introduce post-synthetic masking and chemical activation of guide RNA (gRNA) to controlling CRISPR systems. An RNA structure profiling probe (2-azidomethylnicotinic acid imidazolide) is used. Moreover, we accomplish conditional control of gene editing in live cells. This proof-of-concept study demonstrates promising potential of chemical activation of gRNAs as a versatile tool for chemical biology.

Date: 2020
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DOI: 10.1038/s41467-019-13765-3

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