Polydiacetylene-based ultrastrong bioorthogonal Raman probes for targeted live-cell Raman imaging

Sidan Tian, Haozheng Li, Zhong Li, Huajun Tang, Mingming Yin, Yage Chen, Shun Wang, Yuting Gao, Xiangliang Yang, Fanling Meng (), Joseph W. Lauher (), Ping Wang () and Liang Luo ()
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Sidan Tian: Huazhong University of Science and Technology
Haozheng Li: Huazhong University of Science and Technology
Zhong Li: Stony Brook University
Huajun Tang: Huazhong University of Science and Technology
Mingming Yin: Huazhong University of Science and Technology
Yage Chen: Huazhong University of Science and Technology
Shun Wang: Huazhong University of Science and Technology
Yuting Gao: Huazhong University of Science and Technology
Xiangliang Yang: Huazhong University of Science and Technology
Fanling Meng: Huazhong University of Science and Technology
Joseph W. Lauher: Stony Brook University
Ping Wang: Huazhong University of Science and Technology
Liang Luo: Huazhong University of Science and Technology

Nature Communications, 2020, vol. 11, issue 1, 1-9

Abstract: Abstract Live-cell Raman imaging based on bioorthogonal Raman probes with distinct signals in the cellular Raman-silent region (1800–2800 cm−1) has attracted great interest in recent years. We report here a class of water-soluble and biocompatible polydiacetylenes with intrinsic ultrastrong alkyne Raman signals that locate in this region for organelle-targeting live-cell Raman imaging. Using a host-guest topochemical polymerization strategy, we have synthesized a water-soluble and functionalizable master polydiacetylene, namely poly(deca-4,6-diynedioic acid) (PDDA), which possesses significantly enhanced (up to ~104 fold) alkyne vibration compared to conventional alkyne Raman probes. In addition, PDDA can be used as a general platform for multi-functional ultrastrong Raman probes. We achieve high quality live-cell stimulated Raman scattering imaging on the basis of modified PDDA. The polydiacetylene-based Raman probes represent ultrastrong intrinsic Raman imaging agents in the Raman-silent region (without any Raman enhancer), and the flexible functionalization of this material holds great promise for its potential diverse applications.

Date: 2020
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DOI: 10.1038/s41467-019-13784-0

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