Microparticle traction force microscopy reveals subcellular force exertion patterns in immune cell–target interactions

Daan Vorselen, Yifan Wang, Miguel M. Jesus, Pavak K. Shah, Matthew J. Footer, Morgan Huse, Wei Cai and Julie A. Theriot ()
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Daan Vorselen: Stanford University
Yifan Wang: Stanford University
Miguel M. Jesus: Memorial Sloan Kettering Cancer Center
Pavak K. Shah: Sloan Kettering Institute
Matthew J. Footer: Stanford University
Morgan Huse: Memorial Sloan Kettering Cancer Center
Wei Cai: Stanford University
Julie A. Theriot: Stanford University

Nature Communications, 2020, vol. 11, issue 1, 1-14

Abstract: Abstract Force exertion is an integral part of cellular behavior. Traction force microscopy (TFM) has been instrumental for studying such forces, providing spatial force measurements at subcellular resolution. However, the applications of classical TFM are restricted by the typical planar geometry. Here, we develop a particle-based force sensing strategy for studying cellular interactions. We establish a straightforward batch approach for synthesizing uniform, deformable and tuneable hydrogel particles, which can also be easily derivatized. The 3D shape of such particles can be resolved with superresolution (

Date: 2020
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DOI: 10.1038/s41467-019-13804-z

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