Microparticle traction force microscopy reveals subcellular force exertion patterns in immune cell–target interactions

Daan Vorselen, Yifan Wang, Miguel M. de Jesus, Pavak K. Shah, Matthew J. Footer, Morgan Huse, Wei Cai and Julie A. Theriot ()
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Daan Vorselen: Stanford University, Department of Biochemistry
Yifan Wang: Stanford University, Department of Mechanical Engineering
Miguel M. de Jesus: Memorial Sloan Kettering Cancer Center, Immunology Program
Pavak K. Shah: Sloan Kettering Institute, Developmental Biology Program
Matthew J. Footer: Stanford University, Department of Biochemistry
Morgan Huse: Memorial Sloan Kettering Cancer Center, Immunology Program
Wei Cai: Stanford University, Department of Mechanical Engineering
Julie A. Theriot: Stanford University, Department of Biochemistry

Nature Communications, 2020, vol. 11, issue 1, 1-14

Abstract: Abstract Force exertion is an integral part of cellular behavior. Traction force microscopy (TFM) has been instrumental for studying such forces, providing spatial force measurements at subcellular resolution. However, the applications of classical TFM are restricted by the typical planar geometry. Here, we develop a particle-based force sensing strategy for studying cellular interactions. We establish a straightforward batch approach for synthesizing uniform, deformable and tuneable hydrogel particles, which can also be easily derivatized. The 3D shape of such particles can be resolved with superresolution (

Date: 2020
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DOI: 10.1038/s41467-019-13804-z

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