Quantitative live cell imaging reveals influenza virus manipulation of Rab11A transport through reduced dynein association

Amar R. Bhagwat, Valerie Le Sage, Eric Nturibi, Katarzyna Kulej, Jennifer Jones, Min Guo, Eui Tae Kim, Benjamin A. Garcia, Matthew D. Weitzman, Hari Shroff and Seema S. Lakdawala ()
Additional contact information
Amar R. Bhagwat: University of Pittsburgh School of Medicine
Valerie Le Sage: University of Pittsburgh School of Medicine
Eric Nturibi: University of Pittsburgh School of Medicine
Katarzyna Kulej: The Children’s Hospital of Philadelphia Research Institute
Jennifer Jones: University of Pittsburgh School of Medicine
Min Guo: National Institutes of Health
Eui Tae Kim: The Children’s Hospital of Philadelphia Research Institute
Benjamin A. Garcia: University of Pennsylvania Perelman School of Medicine
Matthew D. Weitzman: The Children’s Hospital of Philadelphia Research Institute
Hari Shroff: National Institutes of Health
Seema S. Lakdawala: University of Pittsburgh School of Medicine

Nature Communications, 2020, vol. 11, issue 1, 1-14

Abstract: Abstract Assembly of infectious influenza A viruses (IAV) is a complex process involving transport from the nucleus to the plasma membrane. Rab11A-containing recycling endosomes have been identified as a platform for intracellular transport of viral RNA (vRNA). Here, using high spatiotemporal resolution light-sheet microscopy (~1.4 volumes/second, 330 nm isotropic resolution), we quantify Rab11A and vRNA movement in live cells during IAV infection and report that IAV infection decreases speed and increases arrest of Rab11A. Unexpectedly, infection with respiratory syncytial virus alters Rab11A motion in a manner opposite to IAV, suggesting that Rab11A is a common host component that is differentially manipulated by respiratory RNA viruses. Using two-color imaging we demonstrate co-transport of Rab11A and IAV vRNA in infected cells and provide direct evidence that vRNA-associated Rab11A have altered transport. The mechanism of altered Rab11A movement is likely related to a decrease in dynein motors bound to Rab11A vesicles during IAV infection.

Date: 2020
References: Add references at CitEc
Citations: View citations in EconPapers (1)

Downloads: (external link)
https://www.nature.com/articles/s41467-019-13838-3 Abstract (text/html)

Related works:
This item may be available elsewhere in EconPapers: Search for items with the same title.

Export reference: BibTeX RIS (EndNote, ProCite, RefMan) HTML/Text

Persistent link: https://EconPapers.repec.org/RePEc:nat:natcom:v:11:y:2020:i:1:d:10.1038_s41467-019-13838-3

Ordering information: This journal article can be ordered from
https://www.nature.com/ncomms/

DOI: 10.1038/s41467-019-13838-3

Access Statistics for this article

Nature Communications is currently edited by Nathalie Le Bot, Enda Bergin and Fiona Gillespie

More articles in Nature Communications from Nature
Bibliographic data for series maintained by Sonal Shukla () and Springer Nature Abstracting and Indexing ().