MAP3Kinase-dependent SnRK2-kinase activation is required for abscisic acid signal transduction and rapid osmotic stress response

Yohei Takahashi, Jingbo Zhang, Po-Kai Hsu, Paulo H. O. Ceciliato, Li Zhang, Guillaume Dubeaux, Shintaro Munemasa, Chennan Ge, Yunde Zhao, Felix Hauser and Julian I. Schroeder ()
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Yohei Takahashi: University of California San Diego
Jingbo Zhang: University of California San Diego
Po-Kai Hsu: University of California San Diego
Paulo H. O. Ceciliato: University of California San Diego
Li Zhang: University of California San Diego
Guillaume Dubeaux: University of California San Diego
Shintaro Munemasa: Okayama University, Tsushima-Naka
Chennan Ge: University of California San Diego
Yunde Zhao: University of California San Diego
Felix Hauser: University of California San Diego
Julian I. Schroeder: University of California San Diego

Nature Communications, 2020, vol. 11, issue 1, 1-12

Abstract: Abstract Abiotic stresses, including drought and salinity, trigger a complex osmotic-stress and abscisic acid (ABA) signal transduction network. The core ABA signalling components are snf1-related protein kinase2s (SnRK2s), which are activated by ABA-triggered inhibition of type-2C protein-phosphatases (PP2Cs). SnRK2 kinases are also activated by a rapid, largely unknown, ABA-independent osmotic-stress signalling pathway. Here, through a combination of a redundancy-circumventing genetic screen and biochemical analyses, we have identified functionally-redundant MAPKK-kinases (M3Ks) that are necessary for activation of SnRK2 kinases. These M3Ks phosphorylate a specific SnRK2/OST1 site, which is indispensable for ABA-induced reactivation of PP2C-dephosphorylated SnRK2 kinases. ABA-triggered SnRK2 activation, transcription factor phosphorylation and SLAC1 activation require these M3Ks in vitro and in plants. M3K triple knock-out plants show reduced ABA sensitivity and strongly impaired rapid osmotic-stress-induced SnRK2 activation. These findings demonstrate that this M3K clade is required for ABA- and osmotic-stress-activation of SnRK2 kinases, enabling robust ABA and osmotic stress signal transduction.

Date: 2020
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DOI: 10.1038/s41467-019-13875-y

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