Non-invasive optical control of endogenous Ca2+ channels in awake mice

Sungsoo Kim, Taeyoon Kyung, Jae-Hee Chung, Nury Kim, Sehoon Keum, Jinsu Lee, Hyerim Park, Ho Min Kim, Sangkyu Lee (), Hee-Sup Shin () and Won Do Heo ()
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Sungsoo Kim: Korea Advanced Institute of Science and Technology (KAIST)
Taeyoon Kyung: Institute for Basic Science (IBS)
Jae-Hee Chung: Korea Advanced Institute of Science and Technology (KAIST)
Nury Kim: Institute for Basic Science (IBS)
Sehoon Keum: Institute for Basic Science (IBS)
Jinsu Lee: Korea Advanced Institute of Science and Technology (KAIST)
Hyerim Park: Korea Advanced Institute of Science and Technology (KAIST)
Ho Min Kim: Institute for Basic Science (IBS)
Sangkyu Lee: Institute for Basic Science (IBS)
Hee-Sup Shin: Institute for Basic Science (IBS)
Won Do Heo: Korea Advanced Institute of Science and Technology (KAIST)

Nature Communications, 2020, vol. 11, issue 1, 1-10

Abstract: Abstract Optogenetic approaches for controlling Ca2+ channels provide powerful means for modulating diverse Ca2+-specific biological events in space and time. However, blue light-responsive photoreceptors are, in principle, considered inadequate for deep tissue stimulation unless accompanied by optic fiber insertion. Here, we present an ultra-light-sensitive optogenetic Ca2+ modulator, named monSTIM1 encompassing engineered cryptochrome2 for manipulating Ca2+ signaling in the brain of awake mice through non-invasive light delivery. Activation of monSTIM1 in either excitatory neurons or astrocytes of mice brain is able to induce Ca2+-dependent gene expression without any mechanical damage in the brain. Furthermore, we demonstrate that non-invasive Ca2+ modulation in neurons can be sufficiently and effectively translated into changes in behavioral phenotypes of awake mice.

Date: 2020
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DOI: 10.1038/s41467-019-14005-4

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