Epigenetic specifications of host chromosome docking sites for latent Epstein-Barr virus

Kyoung-Dong Kim, Hideki Tanizawa, Alessandra De Leo, Olga Vladimirova, Andrew Kossenkov, Fang Lu, Louise C. Showe, Ken-ichi Noma and Paul M. Lieberman ()
Additional contact information
Kyoung-Dong Kim: Chung-Ang University
Hideki Tanizawa: University of Oregon
Alessandra De Leo: The Wistar Institute
Olga Vladimirova: The Wistar Institute
Andrew Kossenkov: The Wistar Institute
Fang Lu: The Wistar Institute
Louise C. Showe: The Wistar Institute
Ken-ichi Noma: University of Oregon
Paul M. Lieberman: The Wistar Institute

Nature Communications, 2020, vol. 11, issue 1, 1-13

Abstract: Abstract Epstein-Barr virus (EBV) genomes persist in latently infected cells as extrachromosomal episomes that attach to host chromosomes through the tethering functions of EBNA1, a viral encoded sequence-specific DNA binding protein. Here we employ circular chromosome conformation capture (4C) analysis to identify genome-wide associations between EBV episomes and host chromosomes. We find that EBV episomes in Burkitt’s lymphoma cells preferentially associate with cellular genomic sites containing EBNA1 binding sites enriched with B-cell factors EBF1 and RBP-jK, the repressive histone mark H3K9me3, and AT-rich flanking sequence. These attachment sites correspond to transcriptionally silenced genes with GO enrichment for neuronal function and protein kinase A pathways. Depletion of EBNA1 leads to a transcriptional de-repression of silenced genes and reduction in H3K9me3. EBV attachment sites in lymphoblastoid cells with different latency type show different correlations, suggesting that host chromosome attachment sites are functionally linked to latency type gene expression programs.

Date: 2020
References: Add references at CitEc
Citations:

Downloads: (external link)
https://www.nature.com/articles/s41467-019-14152-8 Abstract (text/html)

Related works:
This item may be available elsewhere in EconPapers: Search for items with the same title.

Export reference: BibTeX RIS (EndNote, ProCite, RefMan) HTML/Text

Persistent link: https://EconPapers.repec.org/RePEc:nat:natcom:v:11:y:2020:i:1:d:10.1038_s41467-019-14152-8

Ordering information: This journal article can be ordered from
https://www.nature.com/ncomms/

DOI: 10.1038/s41467-019-14152-8

Access Statistics for this article

Nature Communications is currently edited by Nathalie Le Bot, Enda Bergin and Fiona Gillespie

More articles in Nature Communications from Nature
Bibliographic data for series maintained by Sonal Shukla () and Springer Nature Abstracting and Indexing ().