Transcription reinitiation by recycling RNA polymerase that diffuses on DNA after releasing terminated RNA

Wooyoung Kang, Kook Sun Ha, Heesoo Uhm, Kyuhyong Park, Ja Yil Lee, Sungchul Hohng () and Changwon Kang ()
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Wooyoung Kang: Seoul National University
Kook Sun Ha: Korea Advanced Institute of Science and Technology
Heesoo Uhm: Seoul National University
Kyuhyong Park: Seoul National University
Ja Yil Lee: Ulsan National Institute of Science and Technology
Sungchul Hohng: Seoul National University
Changwon Kang: Korea Advanced Institute of Science and Technology

Nature Communications, 2020, vol. 11, issue 1, 1-9

Abstract: Abstract Despite extensive studies on transcription mechanisms, it is unknown how termination complexes are disassembled, especially in what order the essential components dissociate. Our single-molecule fluorescence study unveils that RNA transcript release precedes RNA polymerase (RNAP) dissociation from the DNA template much more often than their concurrent dissociations in intrinsic termination of bacterial transcription. As termination is defined by the release of product RNA from the transcription complex, the subsequent retention of RNAP on DNA constitutes a previously unidentified stage, termed here as recycling. During the recycling stage, post-terminational RNAPs one-dimensionally diffuse on DNA in downward and upward directions, and can initiate transcription again at the original and nearby promoters in the case of retaining a sigma factor. The efficiency of this event, termed here as reinitiation, increases with supplement of a sigma factor. In summary, after releasing RNA product at intrinsic termination, recycling RNAP diffuses on the DNA template for reinitiation most of the time.

Date: 2020
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DOI: 10.1038/s41467-019-14200-3

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