Non-invasive characterization of human bone marrow stimulation and reconstitution by cell-free messenger RNA sequencing

Arkaitz Ibarra (), Jiali Zhuang, Yue Zhao, Neeraj S. Salathia, Vera Huang, Alexander D. Acosta, Jonathan Aballi, Shusuke Toden, Amy P. Karns, Intan Purnajo, Julianna R. Parks, Lucy Guo, James Mason, Darren Sigal, Tina S. Nova, Stephen R. Quake and Michael Nerenberg ()
Additional contact information
Arkaitz Ibarra: Molecular Stethoscope, Inc.
Jiali Zhuang: Molecular Stethoscope, Inc.
Yue Zhao: Molecular Stethoscope, Inc.
Neeraj S. Salathia: Molecular Stethoscope, Inc.
Vera Huang: Molecular Stethoscope, Inc.
Alexander D. Acosta: Molecular Stethoscope, Inc.
Jonathan Aballi: Molecular Stethoscope, Inc.
Shusuke Toden: Molecular Stethoscope, Inc.
Amy P. Karns: Molecular Stethoscope, Inc.
Intan Purnajo: Molecular Stethoscope, Inc.
Julianna R. Parks: Molecular Stethoscope, Inc.
Lucy Guo: Molecular Stethoscope, Inc.
James Mason: Scripps Green Hospital
Darren Sigal: Scripps Green Hospital
Tina S. Nova: Molecular Stethoscope, Inc.
Stephen R. Quake: Stanford University and Chan Zuckerberg Biohub
Michael Nerenberg: Molecular Stethoscope, Inc.

Nature Communications, 2020, vol. 11, issue 1, 1-11

Abstract: Abstract Circulating cell-free mRNA (cf-mRNA) holds great promise as a non-invasive diagnostic biomarker. However, cf-mRNA composition and its potential clinical applications remain largely unexplored. Here we show, using Next Generation Sequencing-based profiling, that cf-mRNA is enriched in transcripts derived from the bone marrow compared to circulating cells. Further, longitudinal studies involving bone marrow ablation followed by hematopoietic stem cell transplantation in multiple myeloma and acute myeloid leukemia patients indicate that cf-mRNA levels reflect the transcriptional activity of bone marrow-resident hematopoietic lineages during bone marrow reconstitution. Mechanistically, stimulation of specific bone marrow cell populations in vivo using growth factor pharmacotherapy show that cf-mRNA reflects dynamic functional changes over time associated with cellular activity. Our results shed light on the biology of the circulating transcriptome and highlight the potential utility of cf-mRNA to non-invasively monitor bone marrow involved pathologies.

Date: 2020
References: Add references at CitEc
Citations: View citations in EconPapers (1)

Downloads: (external link)
https://www.nature.com/articles/s41467-019-14253-4 Abstract (text/html)

Related works:
This item may be available elsewhere in EconPapers: Search for items with the same title.

Export reference: BibTeX RIS (EndNote, ProCite, RefMan) HTML/Text

Persistent link: https://EconPapers.repec.org/RePEc:nat:natcom:v:11:y:2020:i:1:d:10.1038_s41467-019-14253-4

Ordering information: This journal article can be ordered from
https://www.nature.com/ncomms/

DOI: 10.1038/s41467-019-14253-4

Access Statistics for this article

Nature Communications is currently edited by Nathalie Le Bot, Enda Bergin and Fiona Gillespie

More articles in Nature Communications from Nature
Bibliographic data for series maintained by Sonal Shukla () and Springer Nature Abstracting and Indexing ().