A common wild rice-derived BOC1 allele reduces callus browning in indica rice transformation

Kun Zhang, Jingjing Su, Min Xu, Zhihui Zhou, Xiaoyang Zhu, Xin Ma, Jingjing Hou, Lubin Tan, Zuofeng Zhu, Hongwei Cai, Fengxia Liu, Hongying Sun, Ping Gu, Chen Li, Yuntao Liang, Wensheng Zhao, Chuanqing Sun and Yongcai Fu ()
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Kun Zhang: China Agricultural University
Jingjing Su: China Agricultural University
Min Xu: China Agricultural University
Zhihui Zhou: China Agricultural University
Xiaoyang Zhu: China Agricultural University
Xin Ma: China Agricultural University
Jingjing Hou: China Agricultural University
Lubin Tan: China Agricultural University
Zuofeng Zhu: China Agricultural University
Hongwei Cai: China Agricultural University
Fengxia Liu: China Agricultural University
Hongying Sun: China Agricultural University
Ping Gu: China Agricultural University
Chen Li: Rice Research Institute, Guangdong Academy of Agricultural Sciences
Yuntao Liang: Rice Research Institute, Guangxi Academy of Agricultural Sciences
Wensheng Zhao: China Agricultural University
Chuanqing Sun: China Agricultural University
Yongcai Fu: China Agricultural University

Nature Communications, 2020, vol. 11, issue 1, 1-15

Abstract: Abstract Callus browning, a common trait derived from the indica rice cultivar (Oryza sativa L.), is a challenge to transformation regeneration. Here, we report the map-based cloning of BROWNING OF CALLUS1 (BOC1) using a population derived from crossing Teqing, an elite indica subspecies exhibiting callus browning, and Yuanjiang, a common wild rice accession (Oryza rufipogon Griff.) that is less susceptible to callus browning. We show that BOC1 encodes a SIMILAR TO RADICAL-INDUCED CELL DEATH ONE (SRO) protein. Callus browning can be reduced by appropriate upregulation of BOC1, which consequently improves the genetic transformation efficiency. The presence of a Tourist-like miniature inverted-repeat transposable element (Tourist MITE) specific to wild rice in the promoter of BOC1 increases the expression of BOC1 in callus. BOC1 may decrease cell senescence and death caused by oxidative stress. Our study provides a gene target for improving tissue culturability and genetic transformation.

Date: 2020
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DOI: 10.1038/s41467-019-14265-0

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