A rare gain of function mutation in a wheat tandem kinase confers resistance to powdery mildew

Ping Lu, Li Guo, Zhenzhong Wang, Beibei Li, Jing Li, Yahui Li, Dan Qiu, Wenqi Shi, Lijun Yang, Ning Wang, Guanghao Guo, Jingzhong Xie, Qiuhong Wu, Yongxing Chen, Miaomiao Li, Huaizhi Zhang, Lingli Dong, Panpan Zhang, Keyu Zhu, Dazhao Yu, Yan Zhang, Karin R. Deal, Naxin Huo, Cuimin Liu, Ming-Cheng Luo, Jan Dvorak, Yong Qiang Gu, Hongjie Li () and Zhiyong Liu ()
Additional contact information
Ping Lu: The Innovative Academy of Seed Design, Chinese Academy of Sciences
Li Guo: China Agricultural University
Zhenzhong Wang: China Agricultural University
Beibei Li: The Innovative Academy of Seed Design, Chinese Academy of Sciences
Jing Li: Beijing University of Agriculture
Yahui Li: Institute of Crop Sciences, Chinese Academy of Agricultural Sciences
Dan Qiu: Institute of Crop Sciences, Chinese Academy of Agricultural Sciences
Wenqi Shi: Hubei Academy of Agricultural Sciences
Lijun Yang: Hubei Academy of Agricultural Sciences
Ning Wang: The Innovative Academy of Seed Design, Chinese Academy of Sciences
Guanghao Guo: The Innovative Academy of Seed Design, Chinese Academy of Sciences
Jingzhong Xie: The Innovative Academy of Seed Design, Chinese Academy of Sciences
Qiuhong Wu: The Innovative Academy of Seed Design, Chinese Academy of Sciences
Yongxing Chen: The Innovative Academy of Seed Design, Chinese Academy of Sciences
Miaomiao Li: The Innovative Academy of Seed Design, Chinese Academy of Sciences
Huaizhi Zhang: The Innovative Academy of Seed Design, Chinese Academy of Sciences
Lingli Dong: The Innovative Academy of Seed Design, Chinese Academy of Sciences
Panpan Zhang: The Innovative Academy of Seed Design, Chinese Academy of Sciences
Keyu Zhu: The Innovative Academy of Seed Design, Chinese Academy of Sciences
Dazhao Yu: Hubei Academy of Agricultural Sciences
Yan Zhang: China Agricultural University
Karin R. Deal: University of California at Davis
Naxin Huo: USDA-ARS West Regional Research Center
Cuimin Liu: The Innovative Academy of Seed Design, Chinese Academy of Sciences
Ming-Cheng Luo: University of California at Davis
Jan Dvorak: University of California at Davis
Yong Qiang Gu: USDA-ARS West Regional Research Center
Hongjie Li: Institute of Crop Sciences, Chinese Academy of Agricultural Sciences
Zhiyong Liu: The Innovative Academy of Seed Design, Chinese Academy of Sciences

Nature Communications, 2020, vol. 11, issue 1, 1-11

Abstract: Abstract Powdery mildew, caused by Blumeria graminis f. sp. tritici (Bgt), is one of the most destructive diseases that pose a great threat to wheat production. Wheat landraces represent a rich source of powdery mildew resistance. Here, we report the map-based cloning of powdery mildew resistance gene Pm24 from Chinese wheat landrace Hulutou. It encodes a tandem kinase protein (TKP) with putative kinase-pseudokinase domains, designated WHEAT TANDEM KINASE 3 (WTK3). The resistance function of Pm24 was validated by transgenic assay, independent mutants, and allelic association analyses. Haplotype analysis revealed that a rare 6-bp natural deletion of lysine-glycine codons, endemic to wheat landraces of Shaanxi Province, China, in the kinase I domain (Kin I) of WTK3 is critical for the resistance function. Transgenic assay of WTK3 chimeric variants revealed that only the specific two amino acid deletion, rather than any of the single or more amino acid deletions, in the Kin I of WTK3 is responsible for gaining the resistance function of WTK3 against the Bgt fungus.

Date: 2020
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DOI: 10.1038/s41467-020-14294-0

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