Deep tissue space-gated microscopy via acousto-optic interaction

Mooseok Jang (), Hakseok Ko, Jin Hee Hong, Won Kyu Lee, Jae-Seung Lee and Wonshik Choi ()
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Mooseok Jang: Center for Molecular Spectroscopy and Dynamics, Institute for Basic Science (IBS)
Hakseok Ko: Center for Molecular Spectroscopy and Dynamics, Institute for Basic Science (IBS)
Jin Hee Hong: Center for Molecular Spectroscopy and Dynamics, Institute for Basic Science (IBS)
Won Kyu Lee: Korea University
Jae-Seung Lee: Korea University
Wonshik Choi: Center for Molecular Spectroscopy and Dynamics, Institute for Basic Science (IBS)

Nature Communications, 2020, vol. 11, issue 1, 1-11

Abstract: Abstract To extend the imaging depth of high-resolution optical microscopy, various gating operations—confocal, coherence, and polarization gating—have been devised to filter out the multiply scattered wave. However, the imaging depth is still limited by the multiply scattered wave that bypasses the existing gating operations. Here, we present a space gating method, whose mechanism is independent of the existing methods and yet effective enough to complement them. Specifically, we reconstruct an image only using the ballistic wave that is acousto-optically modulated at the object plane. The space gating suppresses the multiply scattered wave by 10–100 times in a highly scattering medium, and thus enables visualization of the skeletal muscle fibers in whole-body zebrafish at 30 days post fertilization. The space gating will be an important addition to optical-resolution microscopy for achieving the ultimate imaging depth set by the detection limit of ballistic wave.

Date: 2020
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DOI: 10.1038/s41467-020-14514-7

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