DNA repair by Rad52 liquid droplets

Roxanne Oshidari, Richard Huang, Maryam Medghalchi, Elizabeth Y. W. Tse, Nasser Ashgriz, Hyun O. Lee, Haley Wyatt and Karim Mekhail ()
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Roxanne Oshidari: University of Toronto
Richard Huang: University of Toronto
Maryam Medghalchi: University of Toronto
Elizabeth Y. W. Tse: University of Toronto
Nasser Ashgriz: University of Toronto
Hyun O. Lee: University of Toronto
Haley Wyatt: University of Toronto
Karim Mekhail: University of Toronto

Nature Communications, 2020, vol. 11, issue 1, 1-8

Abstract: Abstract Cellular processes are influenced by liquid phase separation, but its role in DNA repair is unclear. Here, we show that in Saccharomyces cerevisiae, liquid droplets made up of DNA repair proteins cooperate with different types of DNA damage-inducible intranuclear microtubule filaments (DIMs) to promote the clustering of DNA damage sites and maintain genome stability. Rad52 DNA repair proteins at different DNA damage sites assemble in liquid droplets that fuse into a repair centre droplet via the action of petite DIMs (pti-DIMs). This larger droplet concentrates tubulin and projects short aster-like DIMs (aster-DIMs), which tether the repair centre to longer DIMs mediating the mobilization of damaged DNA to the nuclear periphery for repair. Our findings indicate that cooperation between Rad52 liquid droplets and various types of nuclear filaments promotes the assembly and function of the DNA repair centre.

Date: 2020
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DOI: 10.1038/s41467-020-14546-z

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